IGFl信号通路IGFlIGFlR及AKT在卵巢癌顺铂耐药中表达的研究术-论文.pdf

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1、286中国肿瘤临床2014年第41卷第5期ChinJClinOncol2014，Vo1．41，No．5www．cjco．crlIGF1信号通路IGF1IGF1R及AKT在卵巢癌顺铂耐药中表达的研究术贾美群①陈曾燕①施玲燕②赵斌③吴霞①吴银芳②摘要目的：检测IGF信号通路关键蛋白IGF1、IGF1R及AKT在卵巢癌患者血清及组织中的表达。方法：ATP—TeA法对卵巢癌标本进行药敏试验，ELISA法检测顺铂耐药和敏感组患者血清中IGF1、IGF1R及P—AKT的表达，免疫组织化学检测卵巢癌组织中IGF1、IGF1R、Akt的

2、表达。自患者完成化疗出院后每个月复查CA125，如CA125有异常则行影像学检查，随访时间1年。结果：IGF1、IGF1R72．AKT在卵巢癌顺铂耐药组的表达显著高于敏感组(P=0．0001)，免疫组织化学结果显示顺铂耐药组和敏感组IGF1、IGF1R及AKT的表达相比较差异有统计学意义(P<0．05)。随访顺铂敏感组24例中有18例患者超过1年CA125处于正常值；6例患者超过半年CA125处于正常值；耐药组中有16例患者化疗结束后半年内CA125上升高于正常值，彩超或MRI检查提示有复发病灶。结论：IGF信号通路关键

3、蛋白可能参与卵巢癌顺铂耐药，IGF1可能作为卵巢癌靶向治疗的新靶点。关键词卵巢肿瘤胰岛素生长因子1胰岛素生长因子1受体蛋白激酶B多药耐药蛋白顺铂耐药doi：10．39690．issn．1000—8179．20131238Keyproteinexpressionsintheinsulin-likegrowthfactor-1signalpathwayinvolvedintheresistanceofovariancancertocisplatinMeiqunJIA，ZengyanCHEN，LingyanSHI，BinZHA

4、O。，XiaWU，YinfangWU。CorrespondencetoMeiqunJIA；E—mail：jmq__sk@163．cornDepartmentofGynecology,ScientificInstitutes，DepartmentofPathology,NantongTumorHospital，Nantong226361，China．ThisstudywassupportedbytheMunicipalProjectofScienceandTechnologyforSocialDevelopmentofNa

5、ntong(GrantNo．2010016、．Abstract0hjective：Thisstudyaimedtodetecttheexpressionlevelsofthekeyproteinsinvolvedintheinsulin·-likegrowthfac-torsignalingpathwayofpatientswithovariancancer．Theseproteinsincludeinsulin—likegrowthfactor-1(IGFI)，insulin—likegrowthfactor-1rec

6、eptor(IGF1R)，andproteinkinaseB(AKT)．Methods：OvariancancertissuesweresubjectedtodrugresistancetestsusingtheATP—TCAmethod．IGF1，IGF1R，AKT，andmultidrugresistanceprotein2(MRP2)expressionsweredetectedintheseraofpatientswithovariancancerbyconductingenzyme-linkedimmunoso

7、rbentassay．IGF1，IGF1R，andAKTproteinexpressionsweredetectedinthesurgicalspecimensbyimmunohistochemistry．Patientswereinstructedtomonitortheircancerantigen125(CA125)levelsmonthlyfromthedateapatientwasdischargedtothelastdayofchemotherapy(oruntilchemotherapywascomplet

8、ed)．AcolorDopplerultra-sound，CT，orMRIscanwasrequiredifCA125valueisabnorma1．Thetotalfollow—uptimewasoneyear．Results：IGF1，IGF1R，andAKTexpressionsweresignificantl