β片层阻断肽H102对PAP小鼠脑内ERK信号转导通路的影响-论文.pdf

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1、65oTianjinMedJ，July2014，Vo1．42No．7I3片层阻断肽H102对PAP／]',鼠脑内ERK信号转导通路的影响王冰艳孙凤仙林来祥。徐淑梅m【摘要】目的研究B片层阻断肽H102对PAP双转基因小鼠脑内ERK信号转导通路的激活作用。方法将PAP双转基因小鼠随机分为模型组和给药组，每组10只，设同背景C57BL／6J小鼠为对照组。给药组每日鼻腔给予H102溶液(5．8mg／kg)5儿L，对照组、模型组每日鼻腔给予等体积H102空白辅料溶液。30d后行Morris水迷宫测试。之后采用免疫组化及免疫印迹技术观察小鼠脑组织内RAS、P—MEK及

2、P—ERK蛋白的表达变化。结果(1)Morris水迷宫测试。模型组小鼠学习记忆能力较对照组显著降低，给药组较模型组显著提高(均P<0．05)。(2)免疫组化及免疫印迹检测结果。模型组脑内RAS、P—MEK及P—ERK表达较对照组显著降低，给药组蛋白表达较模型组显著增高(均P<0．01)。结论13J~层阻断肽H102可激活PAP双转基因小鼠脑内ERK信号转导通路，增加神经细胞PAS、P—MEK及P—ERK的含量，改善PAP／ix鼠学习记忆能力。【关键词】阿尔茨海默病；丝裂原激活蛋白激酶激酶类；小鼠，转基因；投药，鼻内；ERK信号转导通路；13J~层阻断肽；RA

3、S；P—MEK；P—ERK【中图分类号】R742【文献标志码】A【DOI】10．39696．issn．0253—9896．2014．07．007TheEffectsof3-SheetBreakerPeptideH102onERKSignalTransductionPathwayinBrainofPAPDoubleTransgenicMiceWANGBingyan，SUNFengxian，LINLaixiang，XUShumeiDepartmentofPhysiology,2InstituteofEndocrinology,TianjinMedicalUniv

4、ersity,Tianjin300070,ChinaXUShumei，E—mail：xushm@tijmu．edu．cn【Abstract】0bjectiveToinvestigatetheactivationofB—sheetbreakerpeptideH102onERKsignaltransductionpathwayinbrainofPAPdoubletransgenicmice．MethodsPAPdoubletransgenicmicewererandomlydividedintomodelgroupandH102treatmentgroup(n=

5、lOforeachgroup)．AgroupofC57BL／6Jmicewiththesamegeneticbackgroundwasservedascontrols．H102(5．8mg／kg)5LwasinfusedbyintranasaladministrationtomiceinH102treatmentgroup，andequalvolumeofblanksolutionofH102(chitosan，BSA)wasgiventomiceincontrolgroupandmodelgroup．Theabilityofspatialreference

6、memorywastestedbyMorriswatermazeafter30daysoftreatment．ThenimmunohistochemistrytestsandWesternblottechniquewereusedtodetectthecontentofRAS，p-MEKandP—ERKproteinsinmousebrain．Results(1)Theabilityoflearningandmemorywassignificantlylowerinmodelgroupthanthatofcontrolgroup．Theabilityofle

7、arningandmemorywassignificantlyimprovedintreatmentgroupthanthatinmodelgroup(P<0．05)．(2)ThecontentsofRAS，P—MEKandP—ERKinmousebrainweresignificantlylowerinmodelgroupthanthoseofcontrolgroup，andtheseproteinex—pressionsweresignificantlyincreasedintreatmentgroupthanthoseinmodelgroupfP<0．

8、01)．Conclusion13一sheetbrea