硒对氧化应激小白鼠肝脏酶活的影响.pdf

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1、J．SltANXIAGRIC．UNIV．(NaturafScienceEdition)岛—目匿l圜嘲学报(自然科学版)2014．34(3OO3132硒对氧化应激小白鼠肝脏酶活的影响李丽娟(山西农业大学信息学院，山西太谷030801)摘要：为研究硒对氧化应激小白鼠肝脏酶活的影响，选取45日龄36只健康昆明品系雄性小白鼠，按体重相近的原则随机分为3组：l组为对照组(硒的水平0。3mg·kg_。W，腹腔注射10mg·kgW的生理盐水)；2组为低硒应激组(硒的水平0．3mg·kgW，腹腔注射D一半乳糖150mg·kgW)；3组高硒应激组

2、(硒的水平0．6mg·kg～‘W，腹腔注射D一半乳糖150mg·kg-1w)。试验结束后，屠宰所有试验小白鼠，摘除眼球采血，分离出肝脏，测定MDA、T—A0C、GSH—PX、LDH、ALT、ASTD的活性。结果表明：与对照组相比，低硒应激组GSHPX活性、T—A0C水平极显著降低，MDA浓度极显著升高；高硒应激组GSH—PX、T—AOC含量差异不显著。低硒应激组与高硒组之间AIT和ALP活性差异显著。与对照组相比，低硒组ALT活性显著，AIP活性极显著增高，高硒应激组IDH、ALT和AIP活性差异不显著，低硒应激组和高硒应激组之

3、间差异显著。0．6mg·kgW硒对D一半乳糖诱导的肝脏损伤有明显的保护作用，这种保护作用可能与硒提高抗氧化酶活性、增强机体的抗氧化防御能力有关。关键词：氧化应激；丙二醛；谷胱甘肽过氧化物酶；乳酸脱氢酶；谷丙转氨酶；硒中图分类号：$852．2文献标识码：A文章编号：167卜8151(2O14)03—0216一O4TheEffectofSeleniumOllLiverEnzymeActivitiesintheOxidativeStressMiceLiLijuan(CollegeofInformation，ShanxiAgricult

4、uralUniversity，TaiguShanxi030801，China)Abstract：Theexperimentwasconductedtostudytheeffectofseleniumontheenzymeactivitiesinliveroftheoxidative-stressedmice．Thisstudyselected36healthykunmingstrainmalemiceattheageof45days．Animalswererandomlydividedintothreegroupsaccordi

5、ngtotheprincipleofsimilarweight：thefirstgroupwasforthecontrolgroup(receiving0．3mg·kg·WSeleniumandintraperitonealinjectionof150mg·kgWD-galactose)，thesecondgroupwasforthelowseleniumstressgroup(receiving0．3mg·kg一WSeleniumandintraperitonealinjectionof150mg·kg～WD-galactos

6、e)，thethirdgroupwasforthehighseleniumstressgroup(receiving0．6mg·kgWSeleniumandintraperitonealinjectionof150mg·_。WD-galactose)．Aftertheestablishmentofanimalmodle，allmicewereslaughtered，andbloodwascollectedbyeyeballremova1．LiverwasquicklyisolatedforthedeterminationofT-

7、AOC，GSH—PX，LDH，ALT，andASTactivitiesandMDAcontent．Theresultswereasfollowed：comparedtoGroup1，theactivitiesofGSHPXandT-AOCdecreased(P<0．01)，andMDAcontentincreasedinGroup2(P％0．01)；inGroup3，comparedtoGroup1，nodifferenceoccurinGSH—PXandT—A()CactivitiesandMDAcontent：thecomp

8、arisonofGroup2togroup3showedthatactivitiveofGSH～PXandT—A()(：decreased(P<0．05)，andMDAcontentincreased(P<0．05)．ComparedwithGroupl，com