透骨草提取物对人结肠癌SWⅢ-C细胞增殖及Bcl-2、Bax蛋白表达的影响.pdf

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1、·264·中国中医药科技2014年5月第2l卷第3期May2014Vo1．21No．3透骨草提取物对人结肠癌SWⅢ一C细胞增殖及Bcl一2、Bax蛋白表达的影响赵彩红曾常茜(大连大学医学院·辽宁省生物有机重点实验室·大连116622)摘要目的：观察透骨草提取物对人结肠癌SWnl—c细胞增殖的影响，探讨透骨草提取物对swm—c细胞增殖影响的分子机制。方法：MTr法检测透骨草提取物对SWⅢ一C细胞增殖的影响，荧光显微镜观察透骨草提取物对SWⅢ一C细胞形态的影响，免疫组化方法检测SWm—c细胞Bcl一2和Bax蛋白的表达。结果：6．25～100／mL透骨草提取物处理的SWⅢ一C细胞增殖抑

2、制率与对照组相比显著增加(P

3、Ⅲ一c细胞增殖eel一2Bax蛋白表达E船ectsofPhrymaLeptostachyaL．var．AsiaticaHaraExtractonProliferationofHumanCo]onCancerSWⅢ一CCellsandBcl一2．BaxExpressionZHAOCai—hong，ZENGChang—qian(MedicalCollegeofDalianUnitersity，Dalian116622)ABSTRACTOblec~ive：ToobservetheefectsofPhr~aleptostaehyaLvar．asiaticaHamextract(PLE)on

4、proliferationofhumancoloncancerSWm—ccellsandtoinvestigatethereliedmolecularmechanism．Methods：TheproliferationinhibitionofPhrymaleptostaehyaLvar．asiaticaHamextractforSWⅢ一CcelswasdetectedwithM"ITassay．ThemorphologyofSWm—cceUswasobservedwithfluorescencemicro-scope．Bcl一2andBaxproteinsofSWm—ccellsw

5、ereexaminedwithimmunohistochemistrytechnique．Results：nleresultsofMTrassayshowedthattheinhibitionratesof6．25—200p,g／mLPhrymaleptostachyaLvar．asiatieaHaraextractforproliferationofSWⅢ一Ccelsincreasedsignificantlytocomparedwiththecon-trolgroup(P<0．05)andthatinhibitionrateforproliferationofSWⅢ一Ccell

6、sincreasedgraduallywiththein—creasingoftheconcentrationofPhrymaleptostachyaLvar．asiaticaHaraextract．ThemedianlethaldoseofPhry-maleptostachyaLvat．asiaticaHaraextractoliSWⅢ一Ccellswas38．36~g／'InLnIeresultsofimmunohisto-chemistrytechniqueshowedthattheexpressionsofBcl-2proteinsinSWⅢ一Ccellsculturedw

7、ith38．36p．g／mLPhrymleptostachyaLvat'．asiaticaHaraextractdecreasedsignificantlytocomparedwithcontrolgroup(P