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1、雷公藤在大鼠1型糖尿病模型中对调节性T细胞Foxp3表达的影响及意义【摘要】目的：研究雷公藤在大鼠1型糖尿病模型中对CD4+CD25+调节性T细胞(TJFoxp3基因表达的影响及意义。方法：将48只大鼠随机分为3组，每组各16只，II、III组采用小剂量多次腹腔注射链腮佐菌素(STZ)的方法制备大鼠1型糖尿病模型，I组为正常对照。III组在成模后给与雷公藤多戒(GTT)灌胃，1次/d,连续3个月。淋巴细胞转化试验(MTT法)检测脾淋巴细胞增殖活性；荧光定量PCR检测外周血及脾淋巴细胞Foxp3mRNA的表达;免疫组化检测淋
2、巴结和脾组织F0XP3蛋白的表达。结果：II、UI组血糖水平高于I组(P<O.01);胰岛中III组淋巴细胞浸润程度较II组减轻;II、III组淋巴细胞增殖活性均较I组升高(P<0.01),用药后皿组较II组降低;Foxp3mRNA.FOXP3蛋白表达水平H、III组均高于I组(P<0.05),III组表达水平较II组有所升高，但差别无统计学意义(P>0.05)o结论：Foxp3+Tr细胞参与了STZ诱导的大鼠1型糖尿病的发生发展；上调Tr细胞Foxp3基因的表达可能为雷公藤治疗1型糖尿病的机制之一
3、。【关键词】CD4+CD25+调节性T细胞Foxp3雷公藤1型糖尿病[Abstract]ATM:ToinvestigatethesignificanceandeffectofGlucosidorumTripterygiitororum(GTT)ontheexpressionsofFoxp3inCD4+CD25+regulatoryTcells(Tr)intype1diabeticratmodel・METHODS:48ratsweredividedevenlyinto3groupsbyrandom.Diabeticmodel
4、wasdevelopedbymultiplelowdoseintraperitonealinjectionofstreptozotociningroupII,III,andgroupIwastreatedasnormalcontrol.Aftertheestablishmentofthemodel,administrationofGTTwasconductedingroupIII,whichwasperformedonceaday,lastingthreemonthes・Lymphocyteproliferationwas
5、detectedbylymphocytetransformationtest(MTTassay)・TheexpressionofFoxp3mRNAwasmeasuredinperipheralbloodandspleenbyusingrea1timePCR・TheexpressionofFoxp3proteininlymphoidnodeandspleenwasdetectedbyimmunohistochemistry.RESULTS:ThebloodglucoselevelingroupTT,ITTwasobvious
6、lyhigherthanthatofcontrolgroup(P&11;0.01),followedwithlymphocyteinfiltratinginpancreaticislets・Theinfi1tratingdegreeingroupITTwasdecreasedthanthatofgroupTI.TheproliferationofspleniclymphocyteingroupII,IIIwasevidentlyincreasedcomparingwiththecontrolgroup(P&It;0.01)
7、.AfteradministrationofGTT,theproliferationingroupIIIwaslowerthanthatingroupII.IngroupII,III,theexpressionsofFoxp3mRNAandproteinincreasedmarkedlycomparedwithcontrolgroup(P&It;0.05)・Moreover,thelevelingroupIIIwasespeciallyenhanced,butcomparedwithgroupII，therewasnosi
8、gnificantlydifferencc(P>0.05).CONCLUSION:Foxp3+Trmayinvolveinthepathogenesyoftype1diabetesindusedbySTZ.GTTdidhavetherapeuticaleffectontype1diabetes,p
