非酒精性脂肪性肝病患者血清中循环microRNAs表达水平及意义.pdf

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1、56·Vd"著·中国肝脏病杂志(申I子版)》2014年第6卷第2期非酒精性脂肪性肝病患者血清中循环microRNAs表达水平及意义高金保，刘霞(榆林市第一医院消化内科，陕西榆林718000)摘要：目的研究microRNAs(miRNAs)在非酒精性脂肪性肝病(NAFLD)患者血清中的表达水平和意义。方法选择NAFLD患者78例(NAFLD组)和非NAFLD人群58例(对照组)。采集入组人群血清，提取总RNA，应用qRT-PCR方法对血清中miR．21、miR34a、miR．122及miR一451表达水

2、平进行检测；分析miRNAs血清表达水平与脂肪变性程度之问的关系。结果两组人群血清中miR一145(尸=3．290)和miR21(P=1．570)表达水平无显著变化；miR一451(P=0．0309)、miRNA一122(P=0．0083)和miR．34a(尸=0．0032)表达水平显著升高。NAFLD组患者根据肝脏脂肪变性程度分为轻度(46例)和重度(32例)，miRNA．122(户=0．0062)和miRNA．34a(P=0．0044)的表达水平随脂肪变性程度的增加而显著升高。结论NAFLD患者较

3、健康人群血清miR一21、miR一34a、miR一122及miR一451表达水平升高，特别是miR．34a和miR．122可以作为NAFLD诊断的一个生物学标志分子。关键词：非酒精性脂肪性肝病；微d~RNA；脂肪肝ExpressionandsignificanceofcirculatingmicroRNAsinnon-alcoholicfattyliverdiseaseGAOJin—bao、LIUXia(DepartmentofGastroenterology,YulinF订stHospitalofS

4、haanxiProvince。Yulin718000，China)Abstract：0bjectiveToinvestigatetheexpressionsandsignificanceofmicroRNAs(miRNAs)inpatientswithnon—alcoholicfatyliverdisease(NAFLD)．MethodsThereare78casesofpatientswithNAFLD(NAFLDgroup)andnonNAFLDpeople58cases(controlgroup

5、)inthestudy．FromserumtotalRNA，qRT-PCRmethodforserummiR一21，miR34a，miR-122andmiR一451weretested，andanalysistherelationshipbetweenmiRNAsserumexpressionlevelsandthedegreeofsteatosiswasanalyzed．ResultsTheserummiR一145=3．290)andmiR21(尸=1．570)expressionlevelhadn

6、osignificantchangeintwogroups；themiR-451(尸0．0309)，miRNA-122(尸=0．0083)andmiR一34(P=0．0032)significantlyincreasedtheexpressionleve1．NAFLDpatientsaccordingtothedegreeofliversteatosisweredividedintomild(46cases)andsevere(32cases)，themiRNA-122=0．0062)andthemi

7、RNA一34a(P=0．0044)expressionlevelsignificantlyincreasedwiththeincreaseofthedegreesofsteatosis．ConclusionsSerummiR一21，miR一34a，miR-122andmiR-122expressionlevelsofNAFLDpatientswerehigherthannon-NAFLDpeople，especiallyinmiR-34aandmiR一122canbeusedasabiological

8、markerofNAFLDinthediagnosis．Keywords：Non—alcoholicfattyliverdisease；MicroRNA；LiversteatosisMicroRNAs(miRNAs)是一类由19～25个核肝病、心脏病、肾病及自身性免疫疾病】。苷酸组成的，高度保守、非编码RNA分子，可以在非酒精性脂肪性肝病(non．alcoholicfattyliver转录后水平对基因表达进行调控，超过30％的人类disease，NAFL