分子检测技术在食品微生物及基因转殖食品之应用

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1、民國70年至89年台灣地區食品中毒案件病因物質分類表專題演講分子檢測技術在食品微生物及基因轉殖食品之應用主講人弘光科技大學民生學院院長曾浩洋教授MolecularDiagnosticTechnique1.ImmunoassaytechniqueRPLA(Reversephaselatexagglutination)ELISA(Enzymelinkedimmunoassay)RIA(Radioimmunoassay)Immuno-PCR(ImmunomagneticbeadPCR)Proteinbioch

2、ip2.GenedetectionmethodDNAprobe:radioactiveandnon-radioactiveprobePCR(Polymerasechainreaction)MultiplexPCRDNAmicroarrayDNAchip3.MoleculartypingtechniquePFGE(Pulsedfieldgelelectrophoresis)RibotypingRAPD(RandomamplifiedpolymorphicDNA)RFLP(Restrictionfragme

3、ntlengthpolymorphism)PCR-ribotypingGeneralAssayProtocol4.EnzymelabelWashdipsticksandplacein1.SampleLysistubescontainingEnzymeAddLysisSolution(s)tosampleConjugateandincubate.tubesandincubatetoreleaserRNAtarget.5.HybridizationWashdipsticksandplaceintubesco

4、ntainingSubstrate-Chromogen.Incubateforcolor2.Hybridizationdevelopment.Discarddipsticks.AddProbeSolutionandincubate.TheDNAProbeshybridizetotheAddStopSolutiontotubes.rRNAtarget.6.DetectionReadabsorbanceat450nmon3.HybridcapturetheGENE-TRAK®Photometer.Place

5、dipsticksintotubesandRecordtheabsorbancereadingincubate.Target-probehybridsfromthedisplay.attachtothedipstick.1/6分子檢測ProductNameFormatDescriptionGENE-TRAK®ListeriaAssay100pcsColorimetricDNAhybridizationGENE-TRAK®ListeriaDLPAssay100pcsColorimetricDNAhybri

6、dizationGENE-TRAK®E.coliAssay100pcsColorimetricDNAhybridizationGENE-TRAK®SalmonellaAssay100pcsColorimetricDNAhybridizationGENE-TRAK®SalmonellaDLPAssay100pcsColorimetricDNAhybridizationGENE-TRAK®Staphylococcusaureus100pcsColorimetricDNAhybridizationAssayG

7、ENE-TRAK®CampylobacterAssay100pcsColorimetricDNAhybridization¢沙門氏菌屬（Salmonellaspp）SalmonellaTyphimurium,S.Enteritidis,S.Typhi,S.Choleraesuis,S.Schwarzengrund,etc食品病原菌與檢測用生物晶片之開展與應用¢大腸桿菌(Escherichiacoli)（1）腸毒性大腸桿菌(EnterotoxigenicE.coli,ETEC)（2）腸出血性大腸桿菌(En

8、terohemorrhagicE.coli,EHEC)（3）腸病原性大腸桿菌(EnteropathogenicE.coli,EPEC)（4）腸侵入性大腸桿菌(EnteroinvasiveE.coli,EIEC)（5）腸凝集性大腸桿菌(EnteroaggregativeE.coli,EaggEC)（6）擴散附著性大腸桿菌（DiffuselyadherentE.coli,DAEC）2/6分子檢測¢金黃色葡萄球菌(Staphylococcusau