资源描述：
《荧光原位杂交检测宫颈脱落细胞hTERC基因实验方法比较观察.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、荧光原位杂交检测宫颈脱落细胞hTERC基因实验方法比较观察STRONG【摘要［/STRONG目的：探讨荧光原位杂交技术检测端粒酶基因(hTERC)实验方法的最佳方案。方法：采集海医附院收治的140例患者的宫颈脱落细胞，分别应用液基取材刷、无菌棉签取材法;生理盐水低渗、TCT低渗、直接取液滴片、TCT直接低渗4种制片方法;水浴和直接消化法；甲酰胺法和共变性法，通过检测宫颈脱落细胞hTERC基因表达成功率,对比实验方法的最佳方案。结果：取材方法中液基取材刷与无菌棉签的细胞数量满意率分别为95,5%.14.3%,2种
2、方法差异有统计学意义(P0.05);生理盐水低渗、TCT低渗、直接取液滴片、TCT直接低渗4种方法杂交成功率分别是80.0%、84.8%.46.2%.82.1%,其中直接取液滴片与其他法差异有统计学意义(P0.05),其他方法Z间比较差异无统计学意义(P>0.05);2种消化法中水浴和直接法杂交成功率分别为88.9%.94.1%,差界无统计学意义(P>O.05),杂信号比例分别为16.7%.45.6%,差异有统计学意义(P0.05);2种变性法中甲酰胺法和共变性法杂交成功率分别为72.3%、96.0
3、%,差异有统计学意义(P0.05)o结论：荧光原位杂交技术检测宫颈脱落细胞hTERC基因时，以液基取材刷取材细胞数量多，TCT低渗方法制片效果最好，水浴法和共变性杂交成功率最高。STRONG【关键词】/STRONG荧光原位杂交；宫颈脱落细胞;TERC慕因[ABSTRACT]Objeclive:ToinvestigatetheoptimalmethodsoffluorescenceinsituhybridizationonhTERCgenedetection.Methods:140sainplesofcervic
4、alexfoliatedcellsweregatheredbyfluidbasebrushsamplingandasepticcottonsamplingintheAffi1iatedHospitaiofHainanMedicalCollege;moviesweremadebyphysiologicalsalineinfiltration,TCTinfiltration,directdroppinganddirectTCTinfiltration.Allexperimentalmethods,bathanddi
5、rectdigestion,formamideanddenaturationlaw,werecoinparedbydetectingthesuccessexpressionofcervixcastoffcellshTERCgene.Results:Insamplingmethods,thesatisfactoryrateofcellquantityinfluidbasebrushandasepticcottonwere95.5%andl4.3%(P0.05);Inhybridizationtechnique,t
6、hesuccessrateof4methodswere80.0%,84.8%,46.2%,82.1%,respectively,coinparedtodirectdroppingmethod,otherthreeinethodswerepreferable(卩0.05)andwithnodifferencesamongthem.Tntwodigestmethods,thesuccessrateofhybridizationwere88.9%and94.1%,respectively,andwithnodiffe
7、rence(P>0.05).Buthybridizationsignalratiowerel6.7%and45.6%,withsignificantdifference(P0.05)•Intwodenaturationmethods,thesuccessratewere72.3%and96.0%,withsignificantdifference(P0・05).Conclusion:DetectinghTERCgenebyfluorescenceinsituhybridization,fluidbaseb
8、rushisbettersamplingofquantitycel1s,TCTinfi1trotionispreferableinmakingmovies,balhandhybridizationvariabilityhavehighersuccessrate・[KEYWORDS]Fluorescenceinsituhybridization;Cervicalexfoliatedcel
