西维来司钠抑制裸鼠肺癌h460细胞胸腔种植瘤生长和转移的机制

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1、现代肿瘤医学2010年08月第l8卷第08期西维来司钠抑制裸鼠肺癌H460细胞胸腔种植瘤生长和转移的机制朱运奎，郭晓斌，李继东，刘卫，马国成MechanismofONO一5046．NainhibitingtumorgrowthandmetastasisoflungcancerinathymicmouseZHUYun—kui，GUOXiao—bin，LIJi—dong，LIUWei，MAGuo—chengDepartmentofRespiratoryDisease，LanzhouGenerlaHospital，LanzhouCommandPLA，Lanzhou730

2、050，China．【Abstract】0bjective：ToevaluatetheinhibitionofONO一5046．Naontumorgrowthandmetastasisoflungcareerofthethoraciccavityimplantmodelinathymicmouse．Methods：H460cellswereinoculatedsubcutaneouslyintothethoraciccavityofathymicmouse，andthemousewererandomizedintoexperimentalgroupandcontr

3、olgroup．Intheexperimentalgroup，ONO一5046．Nawasinjectedintraperitoneallyatadoseof50mgdailyfromdaylaftertumorin—oculation，thecontrolgroupwastreateddailywithequalvolumeofPBSfor28days．Transplantationtumorweights，therateoftumormetastasis，micelifespan，MMP一2ofbloodwereobserved．Results：Therate

4、offormingtumorwas100％，andthethoraciccavityimplantmoelsgraduallyappearedmetastases．Thetransplantationtumorweightsofex—perimentalgroupwerelessthanthatofcontrolgroup；Theaveragesurvivalperiodsofexperimentalgroupswaslongerthanthatofcontrolgroup．TheexpressionofMMP一2ofexperimentalgroupbloodw

5、aslowerthanthatofcontrolgroup．Conclusion：TheseresultsdemonstratethatONO一5046．NacouldinhibitgrowthandmetastasisofH460cellinathymicmouse，andoneoftheimportantmechanismsinvolvesininhibitionoftheMMP一2expression，thethoraciccavityimplantmodelwaseasertocausemetastsis，andprovidedareliableresea

6、rchtoolforstudyingthemechanismsofONO一5046．Nainhibitingtumorgrowthandmetastasisoflungcancer．【Keywords】lungcarcinomamodel；ONO一5046．Na；matrixmetalloproteinase一2；gelatinasezymographyModernOucology2010，18(O8)：1485—1487【摘要】目的：建立裸鼠肺癌胸腔移植模型，观察西维来司钠(SivelestatSodium或ONO一5046，Na)对裸鼠肺癌生长和转移的抑制作用

7、。方法：将H460大细胞肺癌接种于裸鼠左侧胸腔，并随机分成实验组和对照组。分别给予ONO一5046．Na和等体积PBS皮下注射，共28d。测量瘤重、肺癌转移率，测定动物血液中基质金属蛋白酶2(matrixmetalloproteinase一2，MMP一2)的表达情况。结果：裸鼠肺癌胸腔内接种成瘤率100％，并出现远处转移。对照组瘤重均高于实验组(P<0．05)；对照组裸鼠死亡时间均较实验组早；实验组动物血液中MMP一2的表达明显低于对照组，肺外转移小鼠MMP一2的表达高于没有肺外转移小鼠。结论：ONO一5046．Na可抑制肺肿瘤生长和转移，其作用机制之一与抑制MM

8、P一2的表