艾拉莫德对骨关节炎滑膜成纤维样细胞特性的影响

《艾拉莫德对骨关节炎滑膜成纤维样细胞特性的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据史堡凰星题堂盘查2Q堕生!旦蔓!Q鲞箜!塑g堕i!』丛!坚堂!!!：』!!Y!Q堕，!堕!Q，盟!：!·389·艾拉莫德对骨关节炎滑膜成纤维样细胞特性的影响舒强李兴福侯怀水李栋刘花香【摘要】目的观察骨关节炎(oA)滑膜成纤维样细胞(FLS)体外培养时生长、增殖特性，以及艾拉莫德(T一614)对其增殖能力的影响。探讨艾拉莫德的抗炎机制。方法噻唑蓝(MTr)法和流式细胞仪观察OA和滑膜正常者(Ns)膝关节滑膜组织体外培养时FLS增殖能力和细胞周期的变化；用反转录一聚合酶链反应测定FLS的c—fos和COX一2表达情况，检测不同浓度T一614作用下

2、FLS的细胞增殖、细胞周期和基因表达。结果OA和NS的FLS在体外培养时的生长活性、分化能力的差异无统计学意义。高剂量7r-614对两种FLS的细胞存活分数(sF)的抑制作用差异无统计学意义，但与阴性对照组相比差异有统计学意义(P

3、节炎；成纤维样细胞；滑膜；艾拉莫德EffectofT-614onproHferationcharacteristicsoffibroblastlikesynoviaicellsinosteoarth—tis

4、sHUQiang',LIXing弧HOUHuai-shui,LIDong,LIUHua-xiang．*DepartmentofRheumatology,QiluHospital,ShandongUniversity,J；：n锄250012，ChinaCorrespondingauthor：LIXing-fu,Email：xfidi@163．tom

5、【Abstract】ObjectiveToinvestigatetheproliferationcharacteristicsoffibroblastlikesynoviMcells(FLS)inosteoarthritisinvitroandthemechanismoftheimmunosuppressiveeffectofT一614N一(3一formy～lamino一4一OXO一6一phenoxy-4H—chromen一7一y11methanesulfonamideOffthem．MethodsFLSof0Aandnon—inflamedsyno

6、viumfNS)wereculturedandidentifiedinvitrointhepresenceorabsenceofT一614．Afterincu—bation．thesurvivalfraction(SF)ofFLSwasevaluatedbyMTY，cellcyclewasobservedusingfluorescence—activatedcellsorting(FCS)methodandtheexpressionofe-fosandCOX一2IllRNAwasexaminedbyRT—PCRinFLS0f0Apatients．Re

7、sultsNostatisticallysignificantdifferencewasnotedbetweentheOAandNSFLSinproliferationabilityandcellcycle．HighdoseT一614suppressedFLSSFobviouslyinOAandNSsta—tistically(P<0．05)，whereasth'einhibitiondegreewasnotdifferentbetweenthetwokindsofFLS．Theagentinducedcellapoptosisandreducedt

8、heaccumulationofC—fosmRNAinOA—FLSatdose1000ml／L．prolongedG．termandshortenedStermatdose200ml／L．TheexpressionofCOX一2mRNAinOAFLSwassuppressedobviouslybyT-614atdose1000ml／L．Conclusion0AFLSdonotdisplayadistinctactivatedunlimitedviabilitycomparedwithNScells．withoutstimulatedbyproinfl

9、ammatorycytokine讥vitro．HighdoseT一614moderatelyinhibits