壳寡糖对PNPLA3 I148M转基因小鼠肝脏脂质代谢的影响-论文.pdf

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1、6·论著·中国肝脏病杂志(申子版)》2015年第7卷第1期壳寡糖对PNPLA3I148M转基因小鼠肝脏脂质代谢的影响王健，姜曼，辛永宁，宣世英(1．青岛大学医学院附属青岛市市立医院，青岛266021：2．青岛市消化疾病重点实验室，青岛266071；3．青岛市市立医院消化内二科，青岛266011：4．中国海洋大学医药学院，青岛266003)摘要：目的探讨壳寡糖对PNPLA3I148M转基因小鼠肝脏脂质代谢的影响。方法选取5周龄雄性PNPLA3I148M转基因小鼠随机分为模型组和壳寡糖组，以同龄C57BL／6J／b鼠为对照组。壳寡糖组小鼠每日给予200mg／kg

2、壳寡糖，模型组及对照组给予等量生理盐水。16周后，Westernblot检测小鼠肝脏PNPLA3I148M的表达。HE和油红0染色观察小鼠肝脏脂质蓄积情况，并测定肝脏甘油三酯(TG)、总胆固醇(TC)含量。同时检测血清中TG、TC、ALT及AST水平。结果模型组及壳寡糖组小鼠肝脏PNPLA3I148M蛋白表达上调。HE染色示模型组小鼠肝脏发生明显脂肪变性，壳寡糖组小鼠肝细胞内脂肪空泡较少，未见明显脂肪变性；油红0染色示壳寡糖可显著降低转基因小鼠肝细胞内红染脂滴的数量及体积。壳寡糖可减轻PNPLA3I148M基因引起的TG增高，差异有统计学意义(P<0．05)

3、；3组小鼠肝脏TC含量差异无统计学意义(P>0．05)。壳寡糖可降低PNPLA3I148M转基因小鼠血清TG、TC水平，而对ALT和AsT水平无影响。结论壳寡糖能减轻PNPLA3I148M转基因小鼠肝脏脂质沉积，为治疗NAFLD提供新的思路。关键词：寡糖类；脂肪肝，酒精性；小鼠，转基因EffectofehitosanoligosaeeharideonhepaticlipidmetabolismofPNPLA3I148MtransgeniemiceⅥrANGJian‘，JIANGMan，XINYong—ning，xUANShi·ying．QingdaoUniv

4、ersityMedicalCollegeAffiliatedQingdaoMunicrpalHospita1．Qingdao266021，China；2．DigestiveDiseaseKeyLaboratoryofQingdao，Qingdao266071。China,3．SecondDepartmentofGastroenterology,QingdaoMunicipalHospital,Qingdao266011China,"．OceanUniversityMedicalCollegeofChina,Qingdao266003，China)Abstra

5、ct：OhjeetiveToexploretheefectofchitosanoligosaccharide(cos)onhepaticlipidmetabolismofPNPLA3Il48Mtransgenicmice．MethodsFive-week-malePNPLA3I148MtransgenicmicewererandomlydividedintomodelgroupandCOSgroup．TheC57BL／6Jmicewiththesameagewereselectedascontrolgroup．TheCOSgroupwastreatedwit

6、h200meg(‘d)COS．Thesamevolumeofsalinewasadministratedintotheothertwogroups．After16weeks，theproteinexpressionofPNPLA3I148MintheliverofmicewasdetectedbyWesternblot．ThelipidaccumulationofliverwasobservedbyHEandoilredOstaining．Triglyceride(TG)andtotalcholesterol(Tc)contentinliverswereme

7、asured．Atthesametime，sero_mTG，TC，ALTandASTlevelsweredetermined．Results111eproteinexpressionofPNPLA3I148MinmiceliversofmodelgroupandCOSgroupwasup—regulated．HEstainingshowedthatsignificantfattydegenerationwasobservedinliversofmodelgroupandfewfatvacuoleswithnoobvioussteatosiswasfoundi

8、nCOSgroup．OilredOstainings