富血小板纤维蛋白凝胶析出液对人牙髓细胞体外矿化的影响.pdf

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1、第37卷第3期右江民族医学院学报Vo1．37NO．32015年6月JournalofYoujiangMedicalUniversityforNationalitiesJun．2015富血小板纤维蛋白凝胶析出液对人牙髓细胞体外矿化的影响①何璇，韦维，陈文霞②(广西医科大学附属口腔医院牙体牙髓病科，广西南宁530021E—mail：hexuan269@163．eom)摘要：目的探索富血小板纤维蛋白(platelet-richfibrin，PRF)凝胶析出液对人牙髓细胞(humandentalpulpcells，hDPCs

2、)体外矿化的影响。方法组织块法培养hDPCs。采用Choukroun一步离心法制备PRF凝胶。将新鲜制备的PRF凝胶浸泡于DMEM培养基中，于第7d取析出液。用PRF凝胶析出液孵育hDPCs3d后更换矿化诱导液。采用茜素红染色和RT～PCR检测人牙髓细胞矿化的潜能。结果矿化诱导21d后，茜素红染色观察到实验组有少量钙结节生成，而对照组无钙结节生成；RT—PCR结果显示，实验组hDPCs碱性磷酸酶(ALP)的表达为对照组的1．5倍，差异具有统计学意义(P<0．05)。结论PRF凝胶析出液可促进人牙髓细胞矿化。关键词：富

3、血小板纤维蛋白凝胶；析出液；人牙髓细胞；矿化中图分类号：R322．41文献标识码：A文章编号：1001—5817(2015)03—0349—03doi：10．3969／j．issn．1001—5817．2O15．O3．O1Theeffectofplatelet-richfibringelprecipitateliquidonmineralizationofhumandentalpulpcellsinvitroHeXuan，WeiWei，ChenWenxia(DepartmentofEndodontics，theAff

4、iliatedHospitalofStomatology，GuangxiMedicalUniversity，Nanning530021，Guangxi，ChinaE—mail：hexuan269@163．corn)Abstract：objectiveThisstudywasdesignedtoinvestigatetheeffectofplatelet-richfibringel(PRFge1)precipitateliquidonthemineralizationofhumandentalpulpcells(hDP

5、Cs)invitro．MethodsThehD———PCswereseparatedandculturedbyusingtissueblockculturemethod．PRFgelwaspreparedbyChoukrounSprotocols．ThenewlypreparedPRFgelwasdippedinDMEMculturemedia，theprecipitateliquidofPRFgelwascollectedonday7．hDPCsweretreatedwithmineralizationinduct

6、ionsolution3daysafterbeingincubatedwiththeprecipitateliquidofPRFge1．Thecapacityofmineralizationwasmeasuredbyusingalizarinredstai—ningandRT-PCR．ResultsTwenty—onedaysaftermineralizationinduction，asmallamountofmineral—izednodulesonalizarinredstainingwereobservedin

7、experimentalgroupwhilenomineralizednodulewasob—servedincontrolgroup；RT—PCRrevealedthattheexpressionofalkalinephosphatase(ALP)inexperimentalgroupwas1．5timeshigherthanthatincontrolgroup，comparisonyieldedstatisticaldifference(P<0．05)．ConclusionTheprecipitateliquid

8、ofPRFgelcanacceleratethemineralizationofhDPCs．Keywords：platelet-richfibringel；precipitateliquid；humandentalpulpcells；mineralization组织工程化牙髓再生包括三个基本要素：种子细1材料与方法胞、生物支架和局部诱导微环境口