伐地那非对模拟高原低氧性肺动脉高压大鼠的作用.pdf

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1、256TianjinMedJ，March2015，Vo1．43No．3伐地那非对模拟高原低氧性肺动脉高压大鼠的作用周晓玲，潘磊，马婷婷，郭蕊，王勇摘要：目的探讨伐地那非防治高原低氧性肺动脉高压大鼠的效果及可能机制。方法将3O只大鼠随机分为常压常氧对照组(c组)、低压低氧组(P组)和低压低氧+伐地那非组(v组)，每组10只。采用低压低氧舱模拟海拔5000m的高原环境(大气压50kPa，氧浓度10％)，P组和v组大鼠每日入舱8h，V组大鼠每日人舱前给伐地那非lmg／kg灌胃1次，C组和P组每日给予等剂量蒸馏水灌胃1次，共4周。4周后比较各组大鼠的平均肺动脉压、右心肥厚指数、肺血管

2、显微形态学以及血清NO、内皮素一I(ET一1)水平。结果P组的肺动脉压、右心肥厚指数、N4,动脉管壁厚度占血管外径的百分比(wT％)和管壁面积占血管总面积的百分比(wA％)显著高于c组和V组(P<0．05)，血清NO低于c组和v组<0．05)，ET一1高于c组和v组(P<0．05)。结论伐地那非能降低高原肺动脉高压的肺动脉压力，减轻肺血管及右心重构。关键词：高血压，肺性；磷酸二酯酶抑制剂；一氧化氮；内皮缩血管肽1；高原性肺动脉高压；伐地那非中图分类号：R543．2文献标志码：ADOI：10．3969~．issn．0253—9896．2015．03．009Theefectsof

3、vardenafilonhighaltitudepulmonaryhypertensioninratsZHOUXiaoling，PANLei，MATingting，GUORui，WANGYongDepartmentofGeratology,HypoxiaLaboratory,BeijingShiftanHospital，CapitalMedicalUniversity,Beijing100038，ChinaCorrespondingAuthorE—maihwangyongTO96@aliyun．cornAbstract：ObjectiveToinvestigatetheeff

4、ectofvardenafilonhighaltitudepulmonaryhypertensioninrats，andthepossiblemechanismthereof．MethodsThirtyratswererandomlydividedintothreegroups：controlgroupwithnormal——pres—sureandnormal—oxygen(groupC)，pulmonaryhypertensiongroupwithlow—pressureandlow—oxygen(groupP)，andthegrouptreatedbyvardenafi

5、linlow—pressureandlow—oxygencondition(groupV)．TheratsofgroupPandgroupVwereex—posedtolow——pressureandlow——oxygenconditioninanauto——modulatinghypobaricandhypoxiccabintosimulate5000mhighaltitudeenvironment(airpressure50kPa，oxygenconcentration10％)for8hoursdaily．Vardenafil(1mg／kg)wasgivenbygastr

6、ogavagetoratsingroupVoncedailyfor4weeks，whiletheisodosedistilledwaterwasgivenbygastrogavagetoratsingroupCandgroupP．Themeanpulmonaryarterialpressureandrightventricularmassindexweremeasuredrespectivelyafter4-weektreatment．Morphologicchangesofperipheralpulmonarya~eryweredetectedbylightmicrosco

7、pe．Theserumlevelsofnitricoxide(NO)andendothelin-1(ET一11weredetectedaswel1．ResultsThepulmonaryarterialpressureandrightventricularmassindexweresignificantlyhigheringroupPthanthoseofgroupCandgroupVfP<0．05)．Theratioofvascularmedialwallthicknesstoexternaldiam