资源描述：
《孕妇乙肝免疫球蛋白被动免疫阻断HBV母婴垂直传播作用机.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、现代妇产科进展2002年3月第11卷第2期 ProgObstetGynecol,Mar.2002,Vol.11,No.2·论著·孕妇乙肝免疫球蛋白被动免疫阻断HBV母婴垂直传播作用机理的研究11112刘海英,马玉燕,崔保霞,戴 笙,杨 洁(1.山东大学齐鲁医院妇产科,济南 250012;2.武警山东总队医院) 【摘要】 目的:研究HBV阳性孕妇孕期应用乙肝免疫球蛋白(HBIG)预防HBV宫内感染的作用机理。方法:将78例乙肝表面抗原(HBsAg)阳性孕妇分为两组:预防组30例,于孕28、32、36周肌肉注射HBI
2、G3次,每次200IU;对照组48例,只随访查体不用药。检测母儿血清乙肝标志物(HBVM)和细胞因子IFN2γ,IL212,IL26水平用双抗夹心酶联免疫吸附法(DAS2ELISA),测定HBVDNA含量用荧光定量PCR(FQ2PCR)技术。结果:78例HB2sAg阳性孕妇分娩的新生儿宫内感染10例,宫内感染率为12.82%.HBIG预防组孕妇的胎儿HBV感染率显著低于对照组(P<0.05);预防组新生儿脐血清抗2HBs检出率显著高于对照组(P<0.001);预防组孕妇血清中IFN2γ,IL212水平显著高于对照组
3、(P<0.05),IL26水平、HBVDNA含量则显著低于对照组(P<0.05)。结论:孕妇HBIG被动免疫可有效阻断HBV母婴垂直传播。【关键词】 肝炎病毒,乙型;疾病传播,垂直;免疫球蛋白类+中图分类号:R512.62文献标识码A文章编号:1004-7379(2002)02-0128-03StudyonthemechanismofpassiveimmunizationofhepatitisBimmunoglobulinblockingthehepatitisBvirustothematernal2infanti
4、leverticaltransmissionaction.LiuHaiying,MaYuyan,CuiBaoxia,etal.DepartmentofObstetricsandGynecologyofQiluHospitalofShandongUniversity,Jinan,250012【Abstract】Objective:TostudyonthemechanismofpassiveimmunizationwithhepatitisBimmunoglobulin(HBIG)onthepreventionofHB
5、Vintrauterineinfection.Methods:78casesofHB2sAgpositivepregnantwomenweredividedintotwogroups.Intheprophylaxisgroup,30caseswereinjectedwithHBIGat28,32,36weekduringpregnancy(200IUpertime).Inthecontrolgroup,48caseswerefollowed2upwithoutinjectionofHBIG.Byusingdiant
6、ibodysandwichenzymelinkedimmunosorbantassay(DAS2ELISA)andfluorescencequantitativepolymerasechainreaction(FQ2PCR),theHBVmarker(HBVM),activitiesofIFN2γ,IL212,IL26andHBVDNAloadinthebloodsamplesofmothersandtheirneonatesweretestedrespectively.Results:Theincidenceof
7、HBsAgpositivepregnantwomen’sintrauterineinfectionwas12.82%(10/78).InHBIGprophylaxisgroup,theverticaltransmissionincidenceofthenewbornswaslowerthanthoseincontrolgroup(P<0.05).Thedetectionrateofanti2HBsofprophylaxisgroupwassignificantlyhigherthanthoseincontrolgr
8、oup(P<0.001).Inthepregnantwomenoftheprophylaxisgroup,activitiesofIFN2γandIL212weresignificantlyhigherthanthoseincontrolgroup,buttheIL26activityandHBVDNAloadoftheformergroupwerelowe
