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1、___________________________________________________________________www.paper.edu.cnX生物多样性 2003,11(6):486～490BiodiversityScience不同寄主来源寄生疫霉菌株的遗传变异分析3申 贵 王源超 郑小波(南京农业大学植物保护学院农业部病虫监测与治理重点开放实验室, 南京 210095)摘要:从300条RAPD随机引物中筛选出扩增多态性丰富的13条引物,对寄主来源不同的29个寄生疫霉(Phy2tophthoraparasit
2、ica)菌株进行基因组DNA遗传变异分析。用筛选出的13条引物对供试菌株进行RAPD2PCR扩增,共产生139条RAPD条带,其中133条为多态性条带,多态检测率为95.7%。利用PopGeneVersion1.31软件对供试菌株间的遗传距离进行聚类分析并构建系统树状图,供试29个菌株被划分为5个遗传聚类组,不同菌株间具有丰富的遗传变异。其中寄主为烟草(Nicotianaspp.)、腊梅(Chimonanthuspraecox)、凤尾兰(Yuccagloriosa)和西番莲(Passifloraedulis)的菌株的遗传结构与其寄主来
3、源具有明显的相关性,而寄主为刺槐(Sophorachinensis)和番茄(Lycopersicumesculentum)的菌株与其寄主来源的相关性较小,来源于不同寄主植物的菌株之间遗传距离较远。结果提示在寄生疫霉与其不同寄主植物的长期协同进化过程中,寄主对病原菌的遗传结构具有一定的影响。关键词:寄生疫霉,寄主,遗传相关性,协同进化中图分类号:Q933文献标识码:A文章编号:1005-0094(2003)06-0486-05GeneticvariationamongPhytophthoraparasiticastrainsisolat
4、edfromdifferenthostplants3SHENGui,WANGYuan2Chao,ZHENGXiao2BoKeyLaboratoryofMonitoringandManagementofPlantDiseaseandInsectsofMinistryofAgricul2tureofChina,CollegeofPlantProtection,NanjingAgriculturalUniversity,Nanjing210095Abstract:Genomicfingerprintsof29isolatesofthepat
5、hogenPhytophthoraparasiticafromdifferenthostswereanalyzedbyusing13RAPDprimersselectedfrom300randomprimerswithabundantpolymorphisms.GenomicDNAfromdifferentisolateswereamplifiedwiththe13selectedprimersand139markedbandswereproduced,ofwhich133bandswerepolymorphic,accounting
6、for95.7%ofthetotalbandsproduced.Basedonthegeneticdistance,adendrogramwasconstructedwiththesoftwarePopGeneVersion1.31,andthe29testedisolatesweredividedintofivegeneticgroups.Analysisshowedthatthegeneticvariationwasveryricha2mongthetestedisolates.TheisolatesfromNicotanatab
7、accum(tobacco),N.glutinosa(tobacco),Chimo2nanthuspraecox(wintersweet),Yuccaspp.(beargrass)andPassifloraedulis(passionflower)wereclusteredintoonegroup,andshowedhighrelativitywiththeirhosts.ThesimilarityofLycopersicumesculentum(tomato)isolateswasabout60.9%,andtheycouldbec
8、lusteredintotwodistinctgroups.ThesimilarityofthefiveisolatesfromSophorachinensis(sophora)wasabout66.7%～88.4%,a
