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1、XX大学毕业论文丝胶对糖尿病肾病大鼠肾功能和肾脏达的影响2014年6月25日丝胶对糖尿病肾病大鼠肾功能和肾脏TIMP1表达的影响作者：宋成军郝祥俊陈志宏【摘要】H的观察彩色蚕茧提取物丝胶对糖尿病肾病(DN)人鼠肾功能和金属蛋白酶组织抑制因子1(TIMP1)表达的影响。方法60貝雄性SD大鼠随机分为5组(每组12只)：正常对照组、DN模型组、丝胶治疗组、二甲双弧组和丝胶预防组。除止常对照组外，其他各组大鼠均采用链月尿住菌素(STZ)制糖尿病动物模型成功后，丝胶治疗组大鼠给予丝胶灌胃(2.4g-kg-1-d-l,35d)、二甲双弧组大鼠给予二甲双呱灌胃(55.33mg-kg
2、-1-d-l,35d),丝胶预防组大鼠于注射STZ前给予同等剂量丝胶灌胃35(1。分别检测各组大鼠的血糖、肌聊、尿索氮和24h尿蛋白含量，免疫组化染色观察肾脏TIMP1蛋白的表达。结果与正常对照组大鼠相比，模型组大鼠的血糖、肌附、尿素氮、24h尿蛋白含量和肾脏TIMP1蛋白的表达均明显升高(P0.01);丝胶治疗组、丝胶预防组和二甲双弧组大鼠的血糖、肌酹、尿素氮、24h尿蛋白含量和肾脏TIMP1蛋白的表达均明显低于模型组(P0.01,P0.05),且丝胶治疗组、丝胶预防组与二甲双弧组比较无明显差别(P0.05)o结论丝胶可明显改善DN大鼠的肾功能和降低肾脏TIMP1蛋白
3、的表达，对DN吋肾脏基质纤维化和肾小球结构破坏具有良好的保护和预防保护作用，且作用与二甲双弧相为。【关键词】丝胶;糖尿病;糖尿病肾病;肾功能；TIMP1【Abstract]ObjectiveToobservetheeffectsofcolorsilkcocoonextractionsericineonrenalfiinctionandtissueinhibitorsoftissueinhibitorofmetalloproteinase1(TIMP1)expressioninkidneyofdiabeticnephropathy(DN)rats.Methods60mal
4、eSDratswererandomlydividedinto5groups(n=12):normal,DNmodel,scricinctreatment,metforminandscricincpreventiongroups.Theratsinmodel,scricinctreatment,metforminandscricincpreventiongroupswereallestablishedDNratsmodelbyintraperitoneallyinjectedstreptozotocin(STZ).Bloodglucose>16.7mmol/Lwastak
5、entojudgeiftheratsmodelweresuccessfullyestablished.Aftertheratsmodelweresuccessfullyestablished:theratsinsericinetreatmentgroupwerelavagedwithsericine(2.4g-kg-bd-1,35d).Theratsinmetformingroupwerelavagedwithmetformin(55.33mgkg-ld-1,35d).Theratsinsericinepreventiongroupwerelavagedwiththes
6、amedosesericinefor35dbeforeinjectingSTZ.Thebloodglucose,creatinine,ureanitrogenand24hoursurineproteinofratsineachgroupwererespectivelydetected.ImmunohistochemicalstainingwasusedtoobservetheexpressionofTIMP1inkidney.ResultsComparedwithnormalcontrolrats,thebloodglucose,creatinine,ureanitro
7、gen,24hoursurineproteinandTIMP1expressioninkidneyofmodelratsincreasedobviously(PO.Ol).Thebloodglucose,creatinine,ureanitrogen,24hoursurineproteinandTIMP1expressioninkidneyofratsinsericintreatmentgroup,sericinpreventiongroupandmetformingroupweresignificantlylowerthanthatof
