楂芪降糖汤对糖尿病肾病大鼠肾脏tgf-β1，vegf和nephrin表达的影响

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1、重庆医科大学硕士研究生学位论文THEEFFECTSOFZHAQIJIANGTANGDECOCTIONONTHEEXPRESSIONOFTGF—Pl，VEGFANDNEPHRININTHEⅪDNEYoFRATSWITHDIABETICNEPHRoPATHYABSTRACTObjectiveToinvestigatetheeffectsofZhaqijiangtangDecoctionontheexpressionofTGF一13,、VEGFandnephrininkidneyofratswithdiabeticnephropa

2、thy，andthepossiblemechanismsofitsrenalprotection．Methods60SDrats，8weeksold，2001-209，aweek’Sadaptivefeeding．Thentheratswererandomlyselected8asthenormalgroup(N，n=8)，therestoftheminjectedSTZtoestablishthemodelratsofdiabeticAfter4weeks，themodelratsofDNweredividedbyrand

3、omnumbertableimo5groups：Modelgroup(M，n=10)，Glibenclamidegroup(G，n=lo)，ZhaqijiangtangDecoctionhi8tl—dosegroup(ZG，n=10)，ZhaqijiangtangDecoctionmid—dosegroup(ZZ，n=10)，ZhaqijiangtangDecoctionlow—dosegroup(ZD．n一10)．Thentherelevantdrugsweregivenintragastricallytodifferen

4、tgroupsrespectively．AttheendoflOthweek，takeInnercanthusbloodtomeasuredBUN，Scrbyroutineanalysemethods；takerenaltissuestodetectthepathologicalmorphologychangesandultrastructuralchanges；theexpressionofTGF—plWaS5detectedbyimmunohistochemicalstaining；theexpressionofVEGF

5、wasbyimmtmofluorescencestaining；theexpressionofnephrinmRNAandnephrinproteinweredeterminedbyapplyingRT-PCRand腑stemblotResultsInmodelgroup，BUN，Scrinbloodwerehigherthanthenormalgroup(P<0．05)，renaltissuepathologicalmorphologychangesandultrastructuralchangesweresignific

6、ant，theexpressionofTGF—D，VEGFwassignificantlyhigherthantheNormalgroup(P<0．01)，andtheexpressionofnephrinmRNAandproteinwassignificantlylowerthantheNormalgroup(P<0．01)．Comparewithmodelgroup．othergroupsimprovedsignificantly,especiallyinZhaqijiangtangDecoctionhigh—doseg

7、roupandGlibenclamidegroup(P

8、ndthemechanismsmaybethroughdown-regulationtheexpressionofTGF—p1，VEGF，up—regulatetheexpressionofnephrmmRNAandnephrinproteintoreducegenerationandpr