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1、CAROTENES(Algae)Preparedatthe51stJECFA(1998),publishedinFNP52Add6(1998)supersedingspecificationspreparedatthe44thJECFA(1995),publishedinFNP52Add3(1995).NoADIallocatedatthe41stJECFAin1993.Metalsthandarsenicspecificationsrevisedatthe59JECFA(2002).SYNONYMSNatura
2、lß-carotene,carotenes-natural;INSNo.160a(ii);CI(1975)No.75130;CI(1975)No.40800(ß-Carotene)DEFINITIONCarotenes(algae)areobtainedbysolventextractionofthedriedDunaliellasalina(syn.D.bardawilandD.Kone).Themaincolouringprinciplesaretransandcis-ß-carotenetogetherwi
3、thminoramountsofothercarotenoidssuchasalpha-caroteneandxanthophylls.Besidesthecolourpigments,carotenes(algae)maycontainlipids,naturallyoccurringinthesourcematerial,foodgradevegetableoil,andtocopheroladdedtoretardoxidationofthepigment.Theonlysolventsusedforthe
4、extractionarecarbondioxide,acetone,methanol,propan-2-ol,hexane,ethanolandvegetableoil.Themainarticlesofcommercearesuspensionsinfoodgradevegetable/plantoil.Othersareliquidinfoodgradeoil,dispersioninwaterusingfoodadditiveemulsifier,andasapowderusingapulverizing
5、agentsuchasdextrin.Thisisforeaseofuseandtoimprovestabilityasß-caroteneseasilyoxidize.ClassCarotenoidC.A.S.number7235-40-7ChemicalformulaC40H56(ß-Carotene)Structuralformulaall-trans-ß-CaroteneFormulaweight536.88(ß-Carotene)AssayContentofcarotenes(calculatedasß
6、-carotene)isnotlessthandeclaredDESCRIPTIONRed-browntobrownororangetodarkorangesolid,suspension,liquid,dispersionorpowderFUNCTIONALUSESColourCHARACTERISTICSIDENTIFICATIONSolubility(Vol.4)InsolubleinwaterSpectrophotometryAcyclohexanesolutionofthesample(1in200,0
7、00)showsmaximum(Vol.4)absorptionsat448-457and474-486nmColourreactionAspotofasolutionofthesampleintoluene(about400µg/mlofß-carotene)onafilterpaperturnsblue2-3minafterapplicationofasprayordropof20%solutionofantimonytrichloridesolutionintoluene.PURITYResidualsol
8、ventsNotmorethan50mg/kg,singlyorincombination,ofacetone,hexane,(Vol.4)methanol,ethanolandpropan-2-olTocopherolsNotmorethan0.5%SeedescriptionunderTESTSLead(Vol.4)Notmorethan2mg/kgDetermine
