实验性肝硬化大鼠细胞因子IL18、TNFα、IFNγ的变化及意义【精品论文】.doc

《实验性肝硬化大鼠细胞因子IL18、TNFα、IFNγ的变化及意义【精品论文】.doc》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、实验性肝硬化大鼠细胞因子IL18、TNFaIFNY的变化及意义—作者：小小小作者：党双锁，高宁，程延安，边静，王顺达，孙明珠【摘要】FI的探讨在四氯化碳(CC14)复合因素诱导实验性肝硬化大鼠的形成过程屮，白介素18(IL18)、肿瘤坏死因子a(TNFa)、Y干扰素(IFNY)的变化及意义。方法将80只清洁级雄性SD大鼠随机分为正常对照组和造模2周组、4周组、6周组，每组20只。予以CC14复合因素诱导大鼠肝硕化，并在2、4、6周3个吋间点分别处死6只大鼠。采H]ELISA法检测大鼠血清IL18、TNFa、IFNY和肝脏组织匀浆上清液屮的IL18含量；HE染色观察肝脏的组织病理学变化

2、。结果①CC14复合因素诱导的实验性大鼠，组织病理学观察发现，2周时人鼠肝脏组织细胞肿胀变性，6周时有大量纤维增牛，部分肝组织有假小叶的形成；②随着造模吋间的延长，实验大鼠血清TL18、TNFa>TFNY水平逐渐升高，造模6周组与正常对照组比较差异有统计学意义(P<0.01)；③造模组肝脏组织匀浆屮IL18随肝损害程度的加重而升高，造模6周组与正常对照纽比较差异有统计学意义(1X0.01)。结论在CC14复合因素诱导大鼠肝不更化的形成过程屮，IL18、TNFa、IFNy水平逐渐升高，提示该3种细胞因子与肝硬化的形成及发展有关。【关键词】大鼠；肝硬化；白介素18；肿瘤坏死因子a；Y干扰

3、素ABSTRACT:ObjectiveToinvestigstethechangesofinterleukin18(IL18),tumornecrosisfactora(TNFa)andinterferonY(IFNY)levelsoflivercirrhosisinducedbythecompositefactorsofcarbontetrachloride(CC14)inSDratsandtheirsignificance.MethodsTotally80maleSDratsofcleanclasswererandomlydividedintonormalcontrolgroup

4、(20rats)andmodelgroups,thelatterofwhichwerefurtherdividedintothreegroupsaccordingtothe1engthofadministrationtime,namely,2weekgroup(2wkgroup),4weekgroup(4wkgroup)and6weekgroup(6wkgroup),with20ratsineach.Sixratswerekilledafter2wk,4wkand6wkadministrationtime,respectively.TheratserumlevelsofIL18,TN

5、FuandIFNYandthehepatichomogenatesupernatantofIL18weredetectedbyELISA;pathologicalchangesoflivertissueswereobservedbyHEstaining.Results①Pathologicalobservationrevealedthatinthemodelgroupshepaticcellsdegeneratedandswelledatweek2whilelargeamountsoffibrosisandpseudolobulesofsome1ivertissuesoccurred

6、atweek6.②TheserumlevelsofIL18,TNFuandIFNYweregraduallyincreasedwiththemodelingtime,andtheyweresignificantlyhigherin6weekgroupthaninnormalcontrolgroup(P<0.01).③ThelevelsofhepatichomogenatesupernatantofIL18inthemodelgroupswereelevatedwithliverdamage,andtheyweresignificantlyhigherin6weekgroupthani

7、nnormalcontrolgroup(P<0.01).ConclusionDuringtheformationoflivercirrhosisinducedbycompositefactorsofCC14inrats,IL18,TNFaandIFNYlevelsgraduallyincrease,suggestingthatthethreecytokinesplayacertainroleduringtheoccurrenceof1ivercirrhos