八羟基喹啉铜(Ⅱ)配合物的DNA结合和切割活性及与牛血清白蛋白相互作用.pdf

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1、2O15年7月化学研究第26卷第4期CHEMICALRESEARCHDNAbinding，DNAcleavageandBSAinteractionofabis--8--hydr0xyquin0Iinecopper(II)complexCHENZhanfen，ZHANGShuping，ZHANGJian(H“bPiC0llnboratiInnovationCenterforRareMetalChemistry，HubeiKeyLaboratoryoJPollutantAnalysis&ReuseTechnologyCollegeoJChemistry&ChemicalEngineerin

2、g，HubeiNormalUniversity，Huangshi435002，Hubei，China)Abstract：TheinteractionsbetweenDNA／proteinandthebis-8一hydroxyquinolinecopper(II)complex，CuE8一oHQ]2，wereinvestigatedunderphysiologicalconditionsusingUv_visabsorption，fluorescence，circulardichroism(cD)，andagarosegelelectrophoresis．Theexperimentalr

3、esultssuggestCuE8—0HQ]2couldstronglybindtocalfthymusDNA(CT-DNA)throughanintercalativemodeandinducearemarka—bleconformationalvariationofDN八TheintrinsicbindingconstantKbofCuE8一OHQ]2toDNAis1．15(±0．01)×10L／molandtheapparentbindingconstantKppis4．21×10L／mo1．Furthermore，agar—osegelelectrophoresisreveal

4、ed，inthepresenceofascorbicacid(Vc)undernearlyphysiologicalcondi—tions，CuF8一OHQ]2couldefficientlycleavethesupercoiledpBR322plasmidDNAintoitsnickedandline—arforms，evendegradeintoundetectableminorfragments．Mechanismstudiesdemonstrateddiffusible·()H，H2O2and02arenottheeffectivereactiveoxygenspecies(R

5、OS)inthecleavageprocessmedia—tedbythecomplexandthecopper-oxospeciesmightberesponsibleforthecleavage．Additionally，CuE8一()HQ]2couldalsobindtobovineserumalbumin(BSA)withamediumaffinityandquenchtheintrinsicfluorescenceofBsAthroughasinglestaticquenchingmechanism．AlltheseresultssuggestthatCuE8一()HQ]2e

6、xhibitsbiologicalactionasapotentialchemotherapyagent．Keywords：bis一8一hydroxyquinolinecopper(II)complex；DNAbinding；DNAcleavage；bovineserumalbuminhCCICnumber．0657．39Documentcode：AArticleID：1008—1011(2015)04—0404—13tp／／h八羟基喹啉铜(II)配合物的DNA结合和切割活性XyaC及与牛血清白蛋白相互作用bptC●nK陈战芬，张书萍，张健(湖北师范学院化学与化工学院，污染物分析与资源

7、化技术湖北省重点实验室，_暮稀有金属化学湖北省协同创新中心，湖北黄石435002)摘要：利用紫外吸收光谱、荧光光谱、圆二色光谱(CD)和琼脂糖凝胶电泳等手段研究了八羟基喹啉铜(II)配合物CuE8一OHQ]。与DNA和蛋白质的相互作用．实验结果表明，在生理条件下，CuE8一OHQ]z能通过插人方式较强的与CT—DNA结合，诱导DNA构象的改变．其本征结合常数Kb为1．15(4-o．01)×10L／mol，表观结合常数K为4．21×10。L／mo