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1、Geneticdiversityofmaize(ZeamaysL.)accessionsusinginter-simplesequencerepeat(ISSR)markersVuVanLietNguyenThiThuyLinh2NguyenThiThuy2VuThiBichHanh1PhamQuangTuan1NguyenThiPhuongThao21RiceResearchInstitute,HanoiUniversityofAgriculture,Hanoi,Vietnam;2FacultyofBiotechnology,HanoiUniversityofAgriculture,H
2、anoi,VietnamAbstract:[Objective]ThepresentstudywasconductedtoanalyzethecharacterizationofthegeneticdiversityamonglocalmaizeaccessionsintheNorthmountainregionofVietnamusingISSRmarkers.[Method]TheISSRtechniqueand10primerswereusedtostudygeneticdiversityamong21maizeaccessionsconsistingof12normaland9w
3、axymaizeaccessions,collectedfrom3provincesintheNorthmountainregionofVietnamandLaos.[Result]Atotalof108ISSRfragmentsweredetectedandallofthemwerepolymorphic(100％).Polymorphisminformationcontent(PIC)valuesofISSRprimersrangedfrom0.10-0.39.TheaveragePICvalueforeachprimerwas0.24.Theresolvingpower(Rp)va
4、luerangedfrom14.29-0.48withanaverageof4.48perprimer.AlloftestedISSRprimerswiththeexceptionofISSR-T1generateduniquefragments(presentonlyinoneaccession)in13among21maizeaccessions.BasedonUPGMAanalysis,using70％geneticsimilarityasthecutoff,adendrogramwasconstructedand21maizeaccessionsweregroupedintoth
5、reeclusters.Thesimilaritycoefficientsamongaccessionsrangedfrom0.52-0.90.[Conclusion]ISSRmarkersprovidedinformationongeneticdiversityofmaizeaccessionswhichwasusefulforcollection,conservationandbreedingprogramsinVietnam.ISSRmarkers;geneticdiversity;maizelandraces;UPGMAanalysis;PCRS513A2095-1191(201
6、1)09-1029-06玉米种质材料遗传多样性的ISSR分析VuVanLiet1NguyenThiThuyLinh2NguyenThiThuy2VuThiBichHanh1PhamQuangTuan1NguyenThiPhuongThao21河内农业大学水稻研究所,越南河内；2河内农业大学生物技术系,越南河内摘要：[目的]利用ISSR分子标记分析越南北部山区当地玉米种质材料的遗传多样性。[方法]采用ISSR技术和10个引物对从越南和老挝北部山区3个省收集的21份玉米种质材料即12份普通玉米和9份糯玉米的遗传多样性进行分析。[结果]在21份玉米种质材料中可以检测到108个ISSR片段,其多
7、态性为100％。ISSR引物的多态性信息量值为0.10～0.39,每条引物平均为0.24;分辨力为14.29～0.48,平均每条为4.48。除ISSR-TI以外,所有ISSR引物在13份玉米材料中均产生特异性片段。根据聚类分析结果,使用70％的遗传相似性作为切割点,建立了玉米种质材料系统树,21份玉米种质材料被分为3大类。不同玉米材料的相似系数为0.52～0.90.[结论]IssR标记可提供玉米种质遗传多样性信息,对越南玉米种质材料
