泡桐种质资源遗传多样性的issr研究

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1、repeat(ISSR)markerswasemployedtoanalyzegeneticsimilarityandrelationshipwithinthese45accessionsincludesspecies，varieties，morphologicalvariation，andclones．ofPaulownia，aimingtoprovidescientificbasisfortheC1assincationandidentificationofplantsinPaulownia．Atthes

2、ametime，toprovidereferencesforthePaulowniasystemamticsofintraspeciesandinterspeciesofPaulowniafortunei，aswellasgeneticvariationsandgeneticdiversityamong38provenancesofPaulowniafortuneiwereevaluatedbythesamemeansinthispaper．Inordertooffersomemolecularbiology

3、theoriesonbreedingofeliteprovenanceandplantsandhybridizationbreeding．inPaulowniafortunei．Themainresultsweresummarizedasfollows：(1)ISSR—PCRoptimizationreactingsystemofPaulownia，inatotalvolumeof20plincludedthefollowingcomponents：1xbuffer，templateDNAof40ng，M92

4、+of1．5mmol／L，dNTPofO．30mmol／L，primerof0．41xmol／L，andTaqpolymeraseof1U．(2)TheISSRnngerprintingsof45accessionsingenusPaulowniawereconsrtucted，andprovedthatISSRwereaveryeffectivetoolandrobustmethodinaccessionsdiscriminationinPaulowniaTherewere2independentwayst

5、0identifythese45accessions(uniquebandpattenrsandaeombinationofthebandpattenrsprovidedbydieffrentPrimers．TheresultshowedtheeultivarshaduniqueISSRfingerprinting，(Figuresinbracketsrefertoserialnumberintable2．1)①蜘rtunei(4)：P855—1400bp，P855—850bp，P81O一950bp②跏rge

6、sii(5)：P807—2000bp，P807-850bp，P834—1700bp③MorphologicalvariationtypesofP．toment：P807-960bp，P836—1700bp，⑨Morphologicalvariationtypesof跏rtunei：P811—3000bp，P815—2700bp，P8lO一550bp⑥Morphologicalvariationtypesofe．fortunei4(27)：P815-3000bp⑦ElitetreeofPfargesiil(35

7、)：P8l0-250bp(3)ResultsfromISSRmakerresearchesongeneticdiversityof45plantsinPaulownia．9outofl00primerswerescreened，andtotal109fragmentswereamplifiedofwhich103bandsarepolymorphic．Percentageofpolymorphicloci(PPL)amongdifferentprimerwasbetween80％andl00％．Genetic

8、IdentityisrangingfromO．37andO．85，themeanobservednumberofalleles(№)，themeaneffectivenumberofalleles(Ne)，themeanNei’sgenediversityindex(聊andthemeanShannon’SInformationindex(DofPaulowniawere1．9541，1．5391，