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1、aB-晶状体蛋白促进体外培养人RPE细胞的增殖唐翱t张学东，蒯、丽，沈（400016^重庆医科大学附属第一医院眼科）［摘要I目的测定aB-晶状体蛋白在体夕卜培养人视网膜色素上皮细胞（retinalpigmentepithelialcell,RPE）中的表达,探讨aB-晶状体蛋白对体外培养人RPE纽I胞增殖的影响。方法传彳瞒养人ARPE-19细胞系，仙芟荧光技术检测□B-晶状体蛋口在IME细胞屮的表达。用不同稀释浓度（体枳比分別为1/1011/10'、1/101/10'、1/10）的aB-晶状体蛋口以及其抗体干预相应组（对照组、蛋口组、抗体组）细胞，MH方法检测PI洞时间点各
2、组细胞光密度值，流式细胞技术检测各组细胞不同时间点的周期及凋门情况。结果aB-晶状体蚩白在体外培养人RI〉E细胞质屮表达；18h,浓度为1/10’〜l/l（f时，3组细胞的光密度值羞异有统$［学总义（P<0.05）：蛋口组与对照纽相比，0〜48h蛋白组的G期比例减小，S、&期比例增加，抗体纽与对照组相比G期比例增加，S、G期比例减少：伽时3组之间凋】'細胞的构成比羞异有统引学意义（P<0.05）o结论证实aB-晶状体蛋白在体外培养人1肝细胞质中表迖并对体外培养人RPE细胞増殖起明显促进作用。［关键词］aB-晶状体蛋Lh人视网膜色索上皮细胞：增殖呻图法分类号］aB-crysta
3、llinplayedaroleinpromotingRPEcellsproliferationTangDerong,ZhangXuedong,ChengXiaoli,ShenQiang,LiYingyuan(DepartmentofOphthalmology.FirstAffiliatedHospital,ChongqingMedicalUniversity,Chongqing,400016,Cliina)
4、Abstract
5、ObjectiveTodetenninetheexpressionofaB-crystallininhumanretinalpigmentepithel
6、ialcells(RPE)andtoinvestigatetheeffectsofaB-ciystallinonRPEproliferationculturedinvitro.McthodsARPE-19ce11sweresubculturedandtheexpressionofaB-crystallinwasdetectedusingimmunofluorescencetechnique.ctB-crystallinindifferentconcentrations(l/102,l/103,l/104,l/105,l/106:VA9anditsantibodywereuse
7、dfortreatingdifferentgroupsofcells、thenODvaluesandcellcycletogetlierwithcellapoptosisatdifferenttimesweremeasuredbyMTTassayandflowcytometry,respectively.ResultsoB«ctystallinweretbundtobeexpressedinthecytoplasmofhumanRPEcellsculturedinvitro；Therewerestatisticallysignificance(P<0.05)incellODv
8、aluesamongthethreegroupsat48hwhentheconcentrationusedwasl/104-l/102；TheproportionofG
9、phasedecreasedwhiletheproportionofS,G?phaseincreasedinaB-crystallingroupcomparedtothecontrolgroup(048h);TheproportionofGiphaseincreasedwhiletheproportionofS,G?phasedecreasedinaB-crystallinantibodygroupcompa
10、redtothecontrolgroup(0-48h)；Theconstituentratiosofapoptoticcellsamongthethreegroupswerestatisticallysignificantat48h(P<0.05)・Conclu或onOurstudyverifiedtheexpressionofaB-crystallininthecytoplasmofhumanRPEcellsculturedinvitroandaB-crystallinplayedadistinctn
