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1、FULLPAPERVirologyThePrevalenceofaGroup2CoronavirusinDogsinJapanTakashiKANESHIMA1),TsutomuHOHDATSU1)*,KumiSATOH1),TomomiTAKANO1),KenjiMOTOKAWA2)andHiroyukiKOYAMA1)1)DepartmentofVeterinaryInfectiousDiseases,SchoolofVeterinaryMedicineandAnimalSciences,KitasatoUniversity,Towada,Aomori034–8
2、628and2)ResearchCenterforBiologicals,TheKitasatoInstitute,Kitamoto,Saitama364–0026,Japan(Received7June2005/Accepted16September2005)ABSTRACT.Caninecoronavirus(CCoV)hasbeenreportedtocauseacutediarrheamainlyinyoungpups.CCoVandfelinecoronavirusareclassifiedasgroup1coronaviruses.However,ith
3、asrecentlybeenreportedintheUnitedKingdomthatthegroup2coronavirusgene,whichismorecloselyrelatedtothebovinecoronavirus(BCoV)andhumancoronavirusstrainOC43,hasbeendetectedinrespiratorytracttissuesamplesfromdogswithrespiratorydisease.Inthisstudy,weexaminedtheprevalenceofantibodiestogroup2co
4、ronavirusesindomesticdogsandcatsinJapanbyaneutralizationtestusingBCoV.All104felineserumsampleswerenegative(<1:5)foranti-BCoVantibodies.Incontrast,ofthe898canineserumsamples,160(17.8%)werepositiveforanti-BCoVantibodies,andtheantibodytitersrangedfrom1:5tomorethan1:640,with1:160beingthemo
5、stfrequent.Nocorrelationwasfoundbetweenthetitersoftheanti-BCoVandanti-CCoVantibodiesinthe198serumsamplesofdogswithaknownhistoryofCCoVvaccination.Weamplified,byRT-PCR,group2coronavirus-specifichemagglutination/esterasegenesintheoralswabsofatotalof10youngpupspresentingwithorhav-ingrecove
6、redfromrespiratorysigns,orhavinganti-BCoVantibodies,withtheresultthat2pupswerepositiveforthehemagglutination/esterasegenes.Theseresultsstronglysuggestthatanunknowngroup2coronavirusaswellastheknownenteritis-causingCCoV(group1coronavirus)isprevalentamongdomesticdogsinJapan.KEYWORDS:bovin
7、ecoronavirus,caninecoronavirus,group2coronavirus,respiratorydisease.J.Vet.Med.Sci.68(1):21–25,2006Thecausativeagentofsevereacuterespiratorysyndromefluenzavirus(CPIV)[2],canineadenovirustype2(CAV-2)(SARS)thatoriginatedinAsiaandspreadallovertheworld[3],andthebacteriumBordetellabronchis
