冠状病毒Coronavirusl论文-2001 Canine coronavirus in Australian dogs.pdf

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1、ScientificCaninecoronavirusinAustraliandogsMJNAYLORa,RPMONCKTONb,PRLEHRBACHbcandEMDEANEaTheextenttowhichCCVisimportantasagastroentericObjectiveToestimatethefrequencyofserumantibodiespathogenintheAustraliandogpopulationispresently(IgGandIgM)tocaninecoronavirus(CC

2、V)intheAustralianunknown,althoughCCVandCVLPhavebeenpreviouslydogpopulationandevaluatetheroleofCCVasacausativereportedinAustralia.6-8Inthesereports,electronmicroscopyofagentofgastroenteritis.faecalsampleswasusedasthedetectionmethodwithaDesignAserologicalsurveyofa

3、ntibodiestoCCVamongrelativelysmallnumberofsamples.differentdogpopulations.InthisstudywedeterminetheprevalenceofserumIgGandIgMantibodiestoCCVfromalargernumberofdogssampledProcedureThedevelopmentandcharacterisationofanfromthroughoutAustralia.indirectELISAforthedet

4、ectionofantibodies(IgGandIgM)toCCVwasundertaken.Seracollectedfrombothdiarrhoealandnon-diarrhoealdogsfromvariouspopulationsthroughoutMaterialsandmethodsAustraliaweretestedfortheseantibodiestoCCV.VirusandcellsCCVTN-449strainandCRFKcelllinewereobtainedfromResultsSe

5、rumsamples(1396)collectedfrom1984toFortDodgeLaboratories,Iowa,USA.1998weretestedforthepresenceofIgGantibodiestoCCV.SamplesweredividedintotwocategoriesonthebasisoftheCellculturenumberofdogshousedtogether.ThegroupswereeitheranCRFKcellswerepropagatedin75cm2cell-cul

6、tureflasksatopenpopulationcontainingdogshousedasgroupsofthreeor37oC(Corning,Australia)ingrowthmediumcontainingless,orkennelpopulations.Serafrom15.8%oftheopenEMEM(TraceBiosciences,Australia),2mML-glutamine,populationand40.8%ofkennelleddogswerepositivefor0.05%LAHa

7、nd10%FBSnotheatinactivated(CSLLtd,CCVantibodies.Theprevalenceofantibodiesvariedfromzeroto76%inkennelleddogs.About23%of128dogsParkville).MaintenancemediumformaintainingcellswithoutpositiveforIgGantibodiestoCCVwerealsopositiveforIgMdivisionconsistedofEMEM,2mML-glu

8、tamine,0.05%antibodiestoCCV,indicatingrecentCCVinfection.Ofthoselactalbuminhydrolysateand5%FBSnotheatinactivated.dogsthatwerepresentedwithclinicalsignsofgastroenterit