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1、VeterinaryResearchCommunications,25(2001)327^336#2001KluwerAcademicPublishers.PrintedintheNetherlandsAntibodyTestingAgainstCanineCoronavirusbyImmunoperoxidasePlaqueStaining1234T.Soma,M.Hara,H.IshiiandS.Yamamoto1VeterinaryDiagnosticLaboratory,MarupiLifet
2、echCo.,Ltd,Ikeda,Osaka;2DepartmentofMicrobiology,SchoolofVeterinaryMedicine,AzabuUniversity,3Sagamihara,Kanagawa;SukagawaAnimalHospital,Sukagawa,Fukushima;4DepartmentofImmunology,CollegeofEnvironmentalandHealthScience,AzabuUniversity,Sagamihara,Kanagawa
3、,JapanCorrespondence:VeterinaryDiagnosticLaboratory,MarupiLifetechCo.,Ltd,103Fushiocho,Ikeda,Osaka563^0011,JapanSoma,T.,Hara,M.,Ishii,H.andYamamoto,S.,2001.Antibodytestingagainstcaninecoronavirusbyimmunoperoxidaseplaquestaining.VeterinaryResearchCommuni
4、cations,25(4),327^336ABSTRACTTheapplicationoftheimmunoperoxidase(IP)plaquestainingprocedure(IPtest)tothediagnosisofcaninecoronavirus(CCV)infectionwasinvestigated.TheIPtestdidnotreactwithserafromeither15speci¢cpathogen-free(SPF)dogsor7SPFdogsimmunizedwit
5、hamultivalentvaccine,includingcanineparvovirustype2,caninedistempervirus,canineadenovirustype2,andcanineparain£uenzavirus.TocomparetheIPtestwiththeneutralizingtest(NT),serafrom240healthydogsandfrom3experimentallyCCV-infecteddogswereexamined.All60serapos
6、itiveforNTantibodywerepositiveforIPantibody,andall180seranegativeforNTantibodywerenegativeforIPantibodyinthehealthydogs.TheIPtitresshowedsimilarchangeswithtimeafterCCVinoculationtothoseoftheNTtitresintheexperimentallyinfecteddogs.These¢ndingsindicatetha
7、ttheIPtestspeci¢callydetectedanti-CCVantibodies.WhentheIPtestandNTwerecomparedindogswithdiarrhoeicsigns.2.1%of48seraand20.3%of74sera,whichwereallnegativeforNTantibody,werepositiveforIPantibodyinthedogsofunderoneyearofageandatleastoneyearofage,respective
8、ly.Thedi¡erencebetweentheIPandNTtitres(log10[reciprocalofIPtitre]^log10[reciprocalofNTtitre])forthediarrhoeicdogsofunderoneyearofage(2.350+0.931)wassigni¢cantlylargerthanthatforthehealthydogs(0.982+0.447)(p50.0001),theNTtitrebein
