冠状病毒Coronavirusl论文-2017 High Prevalence of Middle East Respiratory Coronavirus in Young Dromedary Camels in Jordan.pdf

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1、VECTOR-BORNEANDZOONOTICDISEASESSHORTCOMMUNICATIONVolumeXX,NumberXX,2016ªMaryAnnLiebert,Inc.DOI:10.1089/vbz.2016.2062HighPrevalenceofMiddleEastRespiratoryCoronavirusinYoungDromedaryCamelsinJordan12345NeeltjevanDoremalen,ZaidounS.K.Hijazeen,PeterHolloway,BilalAlOmari,

2、ChesterMcDowell,643789DanielleAdney,HaniA.Talafha,JavierGuitian,JohnSteel,NadimAmarin,MarkosTibbo,EhabAbu-Basha,4AhmadM.Al-Majali,4VincentJ.Munster,1,*andJuergenA.Richt5,*AbstractPrevalenceofMiddleEastrespiratorysyndromecoronavirus(MERS-CoV)wasdeterminedin45dromedar

3、ycamelsfromtwogeographicallyseparatedherdsinJordan.Virussheddingwasonlydetectedinswabsobtainedfromtherespiratorytractandprimarilyobservedincamelsyoungerthan3years.MERS-CoVseroprevalenceincreasedwithageofcamels.Bovineandsheepserawereseronegative.Phylogeneticanalysiso

4、fpartialS2clusteredtheJordanianMERS-CoVstrainswithcontemporaryMERS-CoVstrainsassociatedwithnosocomialoutbreaks.Keywords:dromedarycamel,Jordan,MERS-CoV,phylogeny,serologyIntroductiontwolocationsinJordan.Twocamelherdswereidentifiedtostudy(1)atraditionalbedouincamelherd

5、,inwhichcamelsSincetheidentificationofMiddleEastrespiratoryareallowedtograzeandbrowsefreely(Azraq,Zarqa)and(2)syndromecoronavirus(MERS-CoV)asthecausativeamoreconventionalmixedfarmsetting,wherecamelswereagentofafatalcaseofrespiratorytractdiseaseintheKingdomkeptinpenso

6、nonefarm(Ramtha)(Fig.1A).InRamtha,ofSaudiArabia(KSA)in2012,thevirushascaused>1700bloodsamplesfromadultsheepandcattlewereadditionallylaboratory-confirmedcasesofdisease,including>600fatalcollected.Swabsampleswerecollectedinvirustransportcases(WHO2016).ThefirstknownMERS-

7、CoVoutbreakinmedium.RNAwasextractedfromsamplesusingthehumans,diagnosedretrospectively,occurredinJordanin2012QiaAmpViralRNAkit(Qiagen).FivemicrolitersofRNA(Hijawietal.2013).Thedromedarycamelhasbeenidentifiedwasusedinaone-stepreal-timeRT-PCRUpEassayforastheprimaryreser

8、voirofMERS-CoV,anddirectevidenceforMERS-CoVusingtheRotor-GeneÔprobekit(Qiagen).zoonotictransmissionfromcamelshasbeenreportedinKSAPositives