冠状病毒Coronavirusl论文-2017 Assessing the Detection of Middle East Respiratory Syndrome Coronavirus IgG in Suspected and Proven Cases of Middle.pdf

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1、VIRALIMMUNOLOGYOriginalResearchArticleVolume00,Number00,2017ªMaryAnnLiebert,Inc.Pp.1–5DOI:10.1089/vim.2017.0091AssessingtheDetectionofMiddleEastRespiratorySyndromeCoronavirusIgGinSuspectedandProvenCasesofMiddleEastRespiratorySyndromeCoronavirusInfection1,21,31,21,21AbdulkarimAlhetheel,Haif

2、aAltalhi,AhmedAlbarrag,ZahidShakoor,DeqaMohamed,1,21,21,344MalakEl-Hazmi,AliSomily,MazinBarry,MuhammedBakhrebah,andMajedNassarAbstractMiddleEastrespiratorysyndromecoronavirus(MERS-CoV)causessevererespiratorydiseasewithsignificantmortality.TwotestingmethodsarecurrentlyusedforMERS-CoVdiagnost

3、ics:nucleicaciddetection(thegoldstandard)andserologicalanalysis.Inthisstudy,weevaluatedthedetectionofMERS-CoV-IgGinsuspectedandprovencasesofMERS-CoVinfection.Weenrolled174patients:113hadrespiratorysymptoms/suspectedMERS-CoVinfection,31hadconfirmedinfluenzaAorBinfection,23hadarecentconfirmedME

4、RS-CoVinfection,and7hadconfirmedMERS-CoVinfection1yearbefore.AllunderwentMERS-CoVRNAandMERS-CoV-IgGtesting.ThirtypatientswerefoundtobeMERS-CoVRNApositive;however,duringsero-logicalanalysis,only6(3.4%)patientswerepositiveforMERS-CoV-IgG,1(0.6%)patientwasequivocal,and167(96%)patientswerenegat

5、ive.Amongtheserologicalpositives,fourwererecentlyMERS-CoVRNApositiveandtwowereMERS-CoVRNAnegative.Nocross-reactivitytoinfluenzaAorBwasdetected.Basedonthelackofcorrelationbetweennucleicacidandserologicalanalysis,weconcludethatMERS-CoV-IgGtestingmaynotbesuitablefordiagnosingacuteinfectionores

6、timatingitsprevalenceduringanoutbreak.Inaddition,ourfindingsshowthatMERS-CoV-IgGmaynothavesignificantvalueindeterminingdiseaseseverityorprognosis.Keywords:MERS-CoV-IgG,MERS-CoVRNA,MERSdiagnostictestingIntroductionparedwithsevereacuterespiratorysyndromecoronavirus(SARS-CoV)(3,10),ithasbeenpro

7、posedthatMERS-CoV,Coronavirusesareagroupofpositive-sense,single-afterfurtheradaptationandevolutioninhumans,mayacquirestrandedRNA-envelopedvirusescapableofinfectingandincreasedhuman-to-humantransmissibilityandhavethepo-causingdiseasesbothinhumansandinseveralani