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1、JournalofGeneralVirology(2006),87,3349–3353DOI10.1099/vir.0.82065-0Inter-andintra-variantgeneticheterogeneityofShortCommunicationhumancoronavirusOC43strainsinFrance1111AstridVabret,JuliaDina,ThomasMourez,Ste´phanieGouarin,121Joe¨llePetitjean,SylvievanderWerfa
2、ndFranc¸oisFreymuth1LaboratoryofVirology,UniversityHospitalofCaen,AvenueGeorgesClemenceau,CorrespondenceAstridVabret14033Caencedex,Francevabret-a@chu-caen.fr2LaboratoiredeGe´ne´tiqueMole´culairedesVirusRespiratoires,InstitutPasteur,25–28rueduDocteurRoux,75015
3、Paris,FranceHumancoronavirusOC43(HCoV-OC43)causesacute,self-limitedrespiratoryinfections.Acloserelationshipbetweenbovinecoronaviruses(BCoVs)andHCoV-OC43hasrecentlybeendemonstrated.Thisstudyincludessevenclinical,non-cellculture-adapted,contemporaryHCoV-OC43str
4、ainsdetectedinFrancein2003.ByusingRT-PCRandclonalsequencingoftheS1geneofHCoV-OC43,theinter-variantheterogeneityoftheHCoV-OC43circulatingstrainswasstudiedandtheintra-variantdiversitywasassessedbyinvestigationofaquasispeciescloud.Thispaperbringstotheforefrontah
5、ighgeneticdiversityofcirculatingHCoV-OC43variants.Geneticallydifferentgroupsaredefinedamongthevariantsdescribedinthisstudy.Oneofthesevariantsholdscharacteristicsofanoutlierandpresentsadeletionof12nt,alsofoundinBCoVstrains.Moreover,thepresenceofHCoV-OC43asaquas
6、ispeciescloudinvivoduringanacuteReceived23March2006respiratory-tractillnesswasdiscovered.Ithasalsobeenrevealedthatquasispecies-cloudsizesareAccepted30June2006similarforthetwoviralpopulationstested.Coronaviruses(CoVs)occupyanimportantpositionin(2005a)submitted
7、toGenBank(accessionno.AY391777)virology,beingnotonlythecauseofthesevereacutethecompletegenomesequenceoftheHCoV-OC43respiratorysundrome(SARS)outbreakthefirsthumanprototypestrain(ATCCVR-759)isolatedin1967.Theseemergentinfectiousdiseaseofthe21stcenturybutalsoaaut
8、horsdemonstratedahighrateofsimilaritywithbovinesignificantpathogeninvolvedinmanyworldwiderespira-coronaviruses(BCoVs)andpostulatedthatHCoV-OC43tory-tractinfections.CoVshavebeenfoundinmanya
