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1、ArchivesofVirologyArchivesofVirology78,167--175(1983)~)bySpringer-Verlag1983GeneticHeterofeneityofMurineCoronavirusesByM.M.C.LA~I,J.O.FLE~IIha1,ST.A.STOHLN[AN1,andK.FvJJivv~am@1DepartmentsofMicrobiologyandNeurology,UniversityofSouthernCalifornia,SchoolofMedicine,LosAngeles,Californi
2、a,U.S.A.,and2Departmenf,ofVeterinaryPathology,SchoolofVeterinaryScience,UniversityofTokyoTokyo,JapanWith3FiguresAcceptedAugust3,1983SmnmarySeveralmousehepatitisviruses(MHV)withdifferentpathogenicitywerestudiedbyotigonucleotidefingerprinting.Twostrains,MHV-KandMHV-D,whichwereisolated
3、inJapanand,whichcauseanaplasiaandnecrosisofbonemarrowanddiarrhea,respectively,werefoundtobecloselyrelatedtoMHV-A59,theprototypeMHV.TwootherMHVstrains,isolatedfromnudemice,werefoundtohavedivergedextensivelyfromtheknownMHVstrains.TheMHVsisolatedfromseparateclonedneuroblastomacelllines
4、persistentlyinfectedwithJI-IMstrainwerealsofoundtohavedivergedmoremarkedlythanthecorrespondingvirusmaintainedundertheconditionsoflyricinfection.GeneticdivergenceduringpersistentinfectionmaybeoneofthemechanismsbywhichtheMHVdiverges.IntroductionMousehepatitisvirus(MHV),amemberoftheCor
5、onaviridae,isanP~NAviruscontainingasingle-strandedRNAgenomeof5.4×10amolecularweight(12).Ithasbeenisolatedfrommiceinmanypartsoftheworldandhasbeenassociatedmainlywithhepatitisandencephalitis(2,21).ItrepIieatesinavarietyoftissueculturecellsofrodentorigin,includingfibroblastsandneuronal
6、cells(15).MHVinfectioninvitrogenerallyresultsinthelysisofinfectedcells,butcanalsoleadtopersistentinfection(3,7,19).Innature,thevirussurvivesbyestablishinglatentinfectionsinthegastrointestinaltractofmice(21).Virusesofthisgrouphavealsobeenshowntocauseentericinfectionsandwastingdisease
7、sinnude12Arch.ViroL78/3-4168M.M.C.LAI,J.0.I~LE~-VIII~G,ST.A.S~OI~LIVlA~,andK.FUZlWAI~&:mice(20).Thus,thevariousstrainsofmousehepatitisvirusexhibitawiderangeofpathogenicpotentials.WehavepreviouslystudiedseveralMHVstrainsbyoligonucleotidefinger-printing,andfoundthattheywerewidelydiver
8、gent(t3).However,so
