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1、J.gen.Virol.(1981),54,67-7467PrintedinGreatBritainGeneticVariationofNeurotropicandNon-neurotropicMurineCoronavirusesByH.WEGE,J.R.STEPHENSON,M.KOGA,HANNAWEGEANDV.TERMEULEN*InstituteforVirologyandImmunobiology,UniversityofWfirzburg,VersbacherStrasse7,8700Wfirzburg,FederalRepublic
2、ofGermany(Aeeepted22December1980)SUMMARYThemurinecoronavirusstrainsMHVJHM,MHV1,MHV2,MHV3andMHVA59weretestedfortheirneurovirulenceinweanlingrats.ThestrainJHMwasfoundtobehighlyneurovirulentforweanlingrats,whereastheotherstrainswerenot,oronlyslightly,neurovirulent.MHV1causednolesi
3、onsinweanlingrats.Theotherstrains(MHV2,MHV3andMHVA59)inducedpredominantlysubclinicalinfectionsinweanlingratsasdemonstratedbyanincreaseofantibodiesandinflammatorylesionsintheliver.Analysisofthesestrainsbycross-neutralizationrevealedvariabledegreesofantigenicrelationshipbetweenth
4、eseviruseswhichwerenotrelatedtotheirneurovirutence.However,whenthesestrainswerecomparedbyanalysingtheTt-RNase-resistantoligonucleotidesofvirionRNA,thehighlyneurovirulentstrainJHMwasfoundtodiffersignificantlyinitsnucleotidesequencefromtheotherless-neurovirulentstrains.INTRODUCTI
5、ONMurinecoronavirusesarewidespreadinnatureandcauseavarietyofdiseasesrangingfrominapparentinfectionstoacutediseasessuchashepatitis,enteritisandencephalomyelitis(Gledhill&Andrewes,1951;Nelson,1952;Dicketal.,1956;Manakeretal.,1961;Baileyetal.,1949;Cheereretal.,1949).Theoriginaliso
6、latesofmurinecoronaviruseswererecoveredfromdifferentmousestrainsand,sofar,animalexperimentstotesttheorgantropismofthesevirusesandtostudythepathogenicmechanismsbywhichthesestrainsinducediseaseshavemainlybeencarriedoutinmice(Lampertetal.,1973;Weiner,1973).Recently,however,ithasbe
7、enshowninweanlingratsthatthemurinecoronavirusJHMinducesdifferenttypesofcentralnervoussystem(CNS)diseasewhichcanbeaccompaniedbymarkeddemyelination(Nagashimaetal.,1978a,b,1979).Thisfindingledustoinvestigatetheorgantropismofanumberofothermurinecoronavirusesinrats.Theantigenicityan
8、doligonucleotidepatternofthevirionRNAoftheseviruseswer
