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1、_ArchivesArchVirol(1996)141:1123-1131Virology©Springer-Verlag1996PrintedinAustriaStructuralandfunctionalanalysisoftheSproteinsoftwohumancoronavirus0C43strainsadaptedtogrowthindifferentcellsBriefReportF.Kiinkel*andG.HerderInstitutftirVirologie,Philipps-
2、Universit~itMarburg,Marburg,FederalRepublicofGermanyAcceptedFebruary6,1996Summary.Thereceptor-bindingactivityofstrainCU(growninMDCKIcells)andofstrainVA(adaptedtoVerocells)ofhumancoronavirusOC43wasanalyzedandcomparedwiththebindingactivityofbovinecoronav
3、irus(BCV)andoftheOC43strainprovidedbytheAmericanTypeCultureCollection(AT).Resultsobtainedwithresialylatederythrocytesindicatedthattheabilityofthevirusestorecognize9-O-acetylatedsialicacidinan~2,6-1inkagedecreasedinthefollowingorder:AT>CU>BCV>VA.Onlymin
4、ordifferenceswereobservedwithrespecttothe~2,3-1inkage.TheaminoacidsequenceoftheSproteinofstrainCUandVAwasderivedfromthenucleotidesequenceoftheclonedgene.StrainVAdifferedfromstrainCUin34positions,18intheS1and16inthe$2subunit.Coronavirusesaremembersofthe
5、familyCoronaviridaethatcompriseslarge,single-strandedRNAviruseswithpositivegenomepolarity.Thevirionsarecomposedofanucleocapsidprotein(N)andtwoorthreeglycoproteins,respect-ively.Theglycoproteinsincludethesurfaceprotein(S),thehemagglutinin-esterase(HE)an
6、dthemembraneprotein(M).ThepresenceofanHEproteinisacharacteristicfeatureofaserologicalsubgroupofcoronavirusesthatincludeshumancoronavirusOC43(HCV-OC43),bovinecoronavirus(BCV),mousehepatitisvirus(MHV)andhemagglutinatingencephalomyelitisvirus(forre-*Prese
7、ntaddress:MacoPharmaInternationalGmbH,Langen,FederalRepublicofGermany.1124F.KiinkelandG.Herrlerviewssee[11,12]).Humancoronavirusesinfectcellsoftheupperrespiratorytractandareknowntobeacausativeagentofcommoncolds.Bycontrast,thecloselyrelatedbovinecoronav
8、iruscausesseverediarrhoeainnewborncalves.TheSproteinisamaintargetoftheimmuneresponsetocoronavirusinfectionsandplaysanimportantroleforthebindingofvirionstocellsurfacereceptorsandforthesubsequentpenetrationbyfusionoftheviralenvelopewithth
