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1、ArchVirol(1988)99:173186VirologyArchives©bySpringer-Verlag1988Identificationofthestructuralproteinsofturkeyentericcoronavirus*s.DeaandP.TijssenCentrederechercheenm6decinecompar~e,InstitutArmand-Frappier,Universit6duQu6bec,Laval-des-Rapides,Qu6bec,CanadaAcceptedDecember31,1987Summary.Coronavir
2、usesintheintestinalcontentsofturkeypoultsfromQuebecflockswithoutbreaksofenteritisweredetectedbyelectronmicroscopy.Fiveisolateswereseriallypropagatedinembryonicturkeyeggsbyinoculationofclarifiedintestinalcontentsintotheamnioticcavity.Viralparticlescontainedintheintestinalcontentsofinfectedembr
3、yoswerepurifiedbydifferentialandisopycniccentrifugationinsucrosegradients.Intactvirionspresentinfractionsofdensities1.18to1.20g/mlwereprecipitatedwithtrichloroaceticacidandtheproteincontentwasanalyzedbysodiumdodecylsulfate-polyacrylamidegelelectrophoresis.Atleastsevenpolypeptideswithmolecular
4、weightsrangingfrom27,000to180,000wereregularlydetectedafterelectrophoresisofSDS-solubilizedvirions.Homologousrabbitantiseratoturkeycoronavirusesandpooledserafromconvalescentturkeysimmunochemicallystainedfivepoly-peptidesofapparentmolecularweightsof95,000,72-75,000,66,000,52,000and27,000.Afurt
5、herpolypeptidewithamolecularweightof140,000appearedintheabsenceof2-mercaptoethanolandprobablyrepresentsadimerofthe66,000polypeptide.OneQuebecisolatedifferedfromtheMinnesotastrainbytwoofitspolypeptides,butnoantigenicvariationscouldbedemonstratedamongstthevariousisolateseitherbyimmuno-electronm
6、icroscopy,hemag-glutinationinhibition,orenzymeimmunoassaysonnitrocellulose.IntroductionTransmissibleenteritisofturkeys(bluecombdisease)isanacute,highlyinfec-tiousdiseaseaffectingturkeysofallages,butespeciallypoults.Theprincipalclinicalsignsareanorexia,waterydroppings,markeddehydratation,weigh
7、tlossand,inextremecases,mortality[26].Thelesionsarelimitedtotheintestinalmucosa[1,28].Intheearly1970's,avirus,thatpossessesthetypicalmorphologyofmembers*ThisstudywaspartlysupportedbytheMedicalResearchCouncilofCanadaandtheQuebecFederationofPou
