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1、Anal.Chem.2004,76,3536-3540PiezoelectricImmunosensorforSARS-AssociatedCoronavirusinSputumBoliZuo,ShanmaoLi,ZhaoGuo,*JinfangZhang,andChuanzhiChenTheAnalyticalCenter,InstituteofChemicalDefenseofPLA,1048#,Beijing,102205,P.R.ChinaApiezoelectricimmunosensorwasdevelopedforthemera
2、sechainreaction(RT-PCR)3-5andfluorescentpolymerasedetectionofSARS-associatedcoronavirus(SARS-CoV)inchainreaction.6,7TheycanidentifytheSARS-CoVinthefirstpart.sputuminthegasphase.HorsepolyclonalantibodyTheyrequireahighspecificitybetweenprimersandtemplatesagainstSARS-CoVwasbou
3、ndontothePZcrystalsurfacebutdonothaveaveryhighsensitivitysothatfalsenegativeresultsinanorderedorientationthroughproteinA.Theantigenarelikely.Ontheotherhand,thesemethodsneedspecificsamplewasatomizedintoaerosolbyanultrasonator,byequipment,costlyreagents,andcomplicatedtechniqu
4、e.TheotherwhichtheantibodyonthecrystalcouldspecificallyadsorboneisbasedonSARSantibodyanalysisinpatientsputum,suchSARSantigenandthechangedmassofcrystalwouldleadastheenzyme-linkedimmunosorbentassaymethod8,9andimmu-afrequencyshift.Afrequencycounterwasemployedtonofluorescenceas
5、say.10Butmethodsrequiremoretime(10-20recordtheadmittancefrequency,andtheplotofchangeddays).Inthisperiod,theviruswouldhavebeentransmittedtofrequencywasdisplayedonthecomputer.Undertheothersoptimizedconditions,thefrequencyshiftswerelinearlyThepiezoelectric(PZ)immunosensorisani
6、mportantbiosen-dependentonantigenconcentrationintherangeof0.6-411sor.In1972,ShonsmadethefirstPZimmunosensortodetectíg/mL.Thedevicehasgoodreproducibility(couldbecowserumIgGantibody.Sincethen,thismethodhasbeenreused100timeswithoutdetectablelossofactivity),importantinbiodetect
7、ionforsensitivity,specificity,simplicity,andstability(theimmunosensorwasstableformorethantwoswiftness.ThePZimmunosensorconsistsofaPZcrystalwithanmonthswhenstoredoversilicagelblueat4-6°C),shortantigenorantibodyimmobilizedonitssurface.Thebiospecificanalyzingtime(lessthan2min)
8、,andspecificity.reactionbetweenthetwointeractivemolecules,oneimmobilizedonthesurfa
