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abundanceinthemusclesasearlyas3daysafterelectroporation,effectsmayoccur.PolIIpromoters,whicharecapableofpreciseandwereobservedthroughoutourstudytoday21.Butonlyafewtranscriptioncontrol,maybeappliedtoeffectmorecontrolledDCcellswerepresentatd
2、ay14andday21.Basedonthosesilencing.TheobjectiveofthisworkwastoassesstheantiHBVobservations,wehypothesizethattheinfiltratingmacrophagesaresilencingefficiencyoftransientlyexpressedshort-hairpinRNAsresponsibleforcross-presentationofantigenfromtransfectedmuscle(shRNA)r
3、egulatedbyaPolIIpromoterandtocomparetheirefficacyfiberstoTcells.Indeed,weobservedTcellsactivationinthedrainingtoequivalentPolIII-derivedtranscripts.Apanelof6shRNAsLNbychemokinesandreceptorscDNAarray,withenhancedregulatedbyU6(PolIII)orCMV(PolII)promotersweredesigned
4、expressionofCXCR-1,CXCR-2,CCR-1,CCR-3,CCR-4,CCR-7,totarget3regionsoftheconservedHBVXopenreadingframe.TheCCR-8,CCR-9andCmkbr1l2genes.CD4+andCD8+TcellswereHepG2.2.15cellline,whichconstitutivelyproducesHBV,andHuh7observedtoappearinthemuscleatasearlyasday5,peakbetweenh
5、umanhepatomacelllineweretransfectedwithplasmidsencodingday7andday14,butdisappearatday21byimmunohistochemistryPolIIorPolIIIshRNAexpressioncassettes.Huh7cellswerealsoanalysis.Fullrecoveryofthemusclefiberswasfoundatday28cotransfectedwithpCH3091orpCH3091-GFPHBVtargetve
6、ctors.afterelectroporation,withcenternucleatedregeneratedmusclecellspCH3091isaplasmidthatencodesallHBVsequences,andinandeliminatedinflammatorycellinfiltrates.pCH3091-GFP,thepreS2-SregionhasbeensubstitutedwithasequenceencodingtheEnhancedGreenFluorescentProtein(EGFP)
7、.552.Single-ChainAntibodiesforViral-BasedTheeffectsoftheshRNAsonHBVgenetranscriptionweredeterminedbymeasuringHBsAg,HBeAg,HBVtranscriptsandPassiveImmuno-TherapyforPrionDisease:EGFP.shRNAsthatarederivedfrombothPolIIandPolIIILinearEpitopeMappingandClearancefrompromote
8、rswerecapableofsignificantinhibitionofHBVgeneThalamicInjectionexpression,althoughPolIII-derivedshRNAswerestrongerinducersCharlesA.Wuertzer,1MarkA
