冠状病毒Coronavirusl论文-1997 Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by administration of egg yolk or.pdf

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1、veterinarymicrobiologyELSEVIERVeterinaryMicrobioLogy58(1997)105-111Passiveprotectionofneonatalcalvesagainstbovinecoronavirus-induceddiarrheabyadministrationofeggyolkorcolostrumantibodypowderYutakaIkemori*,MasashiOhta,KoujiUmeda,FaustinoC.IcatloJr.,Masah

2、ikoKuroki,HideakiYokoyama,YoshikatsuKodamaGhenCorporationImmunologyResearchInstitute,839-1Sano,GifuCity,501-11,JapanReceived12December1996;accepted14May1997AbstractTheprotectiveeffectofeggyolkandcolostrumpowderspreparedfromhensandcowsvaccinatedwithinact

3、ivatedbovinecoronavirus(BCV)antigenwasevaluatedinachallengemodelwithavirulentBCVstrain.TwentythreecalvesfromBCV-freeherdswererandomlydividedintocontrolandseveraltreatmentgroups.Allcalveswereorallychallengedwith1x109TCIDsoofthevirulentKakegawastrainofBCV

4、at24to36hafterbirth.CalvesintreatmentgroupsreceivedeithereggyolkpowderorcowcolostrumcontainingBCVspecificantibodies.Dailytreatmentwiththeseantibodypreparationsstarted6huntil7dayspost-challenge.Controlcalveswhichreceivednoantibodyhadseverediarrheaandalld

5、iedwithin6daysafterinfection.Incontrast,calvesfedmilkcontainingeggyolkorcolostrumwithneutralizationtitersof1:2560or1:10240respectivelyallsurvivedandhadpositiveweightgainunliketheothertreatmentgroups.Theseresultsindicatethattheorallyadministeredeggyolkan

6、dcolostrumpowdersprotectedagainstBCV-induceddiarrheainneonatalcalvesandthattheeggyolkusedprovidedahigherdegreeofprotectioncomparedtocolostrumpowderonatiterbasis.Treatmentwithwholeeggyolkfromimmunizedhensthereforeprovidesamoreefficaciousalternativetothee

7、xistingmethodsofspecificpassiveprotectionagainstBCV.©1997ElsevierScienceB.V.Keywords:Cattle-viruses;Bovinecoronavirus;Passiveimmunization;ImmunoLogy;Eggyolk;Colostrum*Correspondingauthor.Tel.:+81-58-235-7303;fax:+81-58-235-7505.0378-1135/97/$17.00©1997E

8、lsevierScienceB.V.Allrightsreserved.PIIS0378-1135(97)00144-2106Elkemorietal./VeterinaryMicrobiolog358(1997)105-1111.IntroductionBovinecoronavirus(BCV)isanimportantagentofneonatalcalfdiarrheaandisassociatedwithacutediarrheaofadult