资源描述：
《冠状病毒Coronavirusl论文-1993 Coronavirus immunogens.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、VeterinaryMicrobiology,37(1993)285-297285ElsevierSciencePublishersB.V.,AmsterdamCoronavirusimmunogensLindaJ.SaifFoodAnimalHealthResearchProgram,OhioAgriculturalResearchandDevelopmentCenter,TheOhioStateUniversity,Wooster,OHUSA(Accepted14July1993)ABSTRACTCoronavirnses(CV)infe
2、ctavarietyoflivestock,poultryandcompanionanimals.Theybelongtoatleastfiveantigenicgroups.CVcauselocalizedinfectionsoftherespiratoryand/orintestinaltracts,withtheexceptionoffelineinfectiousperitonitisvirus(FIPV)andhemagglutinatingencephalomye-litis(HEV)whichcausesystemicinfec
3、tions.TheenteropathogenicCVinfectthevillousenterocytesresultinginvillousatrophyleadingtomalabsorptivediarrhea.SeveralCV(bovineCV-BCV,porcinerespiratoryCV-PRCV,infectiousbronchitisvirus-IBV)causerespiratorydisease.Currentevidenceindicatesthatprotectionagainstentericandrespir
4、atoryCVinfectionsisme-diatedbypassiveoractiveimmunityattheprimarysiteofCVreplication.Maternalvaccinationapproachestoinducepassiveimmunityincludetheuseofinactivatedandmodifiedliveviralvac-cines.ModifiedlivevirusesandaTsmutantCV(FIPV)arealsousedasoralorintranasalvaccinestoind
5、uceactivemucosalimmunity.Thesuccessofthesevaccinesinthefieldisoftencompromisedbyanumberofpotentialproblems.Coronavirusesarespherical,envelopedviruses,rangingfrom80-160nmindiameterandcontain-ingapositive-strandedRNAgenome.Theypossessprominentsurfacespikesandsomespeciesdis-pl
6、ayafringeofsmallersurfaceprojectionsbelievedtobethehemagglutinin(HE).Coronavirusespossess3to4structuralproteins:thespike(S)glycoprotein(150-200kDa),theintegralmembraneglycoprotein(M;20-30kDa)andthenucleocapsidphosphoprotein(N;43-50kDa).AsubsetofCV(BCV,HEV,turkeyCV)possessat
7、hirdglycoproteinonthevirionsurface,theHE(60-65kDa).Theseproteinscanbequamitatedusingpooledmonoclonalantibodies(mAb)todistinctepitopesofeachproteininELISA.MostresearchhasfocusedontheSproteinasacandidateantigenforCVvaccinessinceitinducesvirusneutralizing(VN)antibodies.However
8、theHEproteinstimulatestheproductionofVNandHEinhibitingantibodiesandtheMproteinindu
