冠状病毒Coronavirusl论文-1993 Induction of protective immunity against coronavirus-induced encephalomyelitis_ Evidence for an important role of C.pdf

《冠状病毒Coronavirusl论文-1993 Induction of protective immunity against coronavirus-induced encephalomyelitis_ Evidence for an important role of C.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、Eur.J.Immunol.1993.23:1757-1761Coronavirusprotein-specificprotectiveimmuneresponseinrats1757EgbertFlory,InductionofprotectiveimmunityagainstMichaelPfleiderer.,AlbertStuhlerandcoronavirus-inducedencephalomyelitis:evidenceHelmutWegeforanimportantroleofCD8+Tcellsinvivo*InstituteofVir

2、ologyandImmunobiology,UniversityofCoronavirusMHV-JHMinfectionsofratsprovideusefulmodelstostudytheWurzburg,Wurzburgpathogenesisofvirus-inducedcentralnervoussystemdisease.ToanalyzetheroleoftheimmuneresponseagainstdefinedMHV-JHMantigens,wetestedtheprotectiveefficacyofvacciniavirus(VV

3、)recombinantsexpressingeitherthenucleocapsid(N)orthespike(S)protein.AstrongprotectionwasmediatedinanimalsbyimmunizationwithrecombinantVVencodingawild-typeSprotein(VV-Swildtype),whereasVVrecombinantexpressingamutantS354~~protein(VV-S354~~)hadnoprotectiveeffect.RecombinantVVencoding

4、Nprotein(VV-N)inducesahumoralandaCD4+Tcellresponse,butdidnotpreventacutediseaseregardlessoftheimmunizationprotocol.Intheseexperiments,challengewithanotherwiselethaldoseofMHV-JHMwasperformedpriortotheinductionofvirus-neutralizingantibodiesandstudieswiththeanti-CD8+monoclonalantibod

5、y,MRCOX8showedthateliminationoftheCD8+subsetofTcellsabrogatestheprotectiveeffect.ThisresultindicatesthatCD8+TcellsprimedbyrecombinantVVexpressingwild-typeSproteinareaprimarymechanismofimmunologicaldefenseagainstMHV-JHMinfectioninrats.1Introductiondiseaseinmiceandrats[8-111.Also,pr

6、otectiveneutraliz-ingantibodiescanbeinducedbyimmunizationofmicewithCoronavirusinfectionsofthecentralnervoussystem(CNS)purifiedSproteinoranSproteindecapeptide[12,131.areinterestingmodelstoanalyzemechanismsofinflamma-Theseresultsimpliedthatthehumoralimmuneresponsetorydemyelinationan

7、dviruspersistence.InparticularthemaybesufficientfortheobservedprotectionagainstmurinecoronavirusstrainMHV-JHMinducesdifferentMHV-JHMinfection.coursesofencephalomyelitisinmiceorrats[l,21.ATcell-mediatedimmunityisalsobelievedtobecrucialforInLewisrats,differentformsofthediseasehavebe

8、enthecontrolofcoronavirusinfectio