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1、QINSTITUTPASTEUR/ELSEVIERRes.!mmunol.Paris19901990,141,141-151CHARACTERIZATIONOFBLOODMONONUCLEARCELLSPRODUC-INGIFNaFOLLOWINGINDUCTIONBYCORONAVIRUS-INFECTEDCELLS(PORCINETRANSMISSIBLEGASTROENTERITISVIRUS)B.CharleyandL.LavenantLaboratoiredeVirologieetd'lmmunologiemoidculaires,
2、INRA,78350Jouy-en-Josas(France)SUMMARYPorcinebloodmononuclearcells(PBMC)wereshowntoproduceinterferon-a(IFNa)followingincubationwithcellsinfectedbyacoronavirus,transmissiblegastroenteritisvirus.Monoclonalantibodies(mAb)withspecificitiesforleukocytesubsetsandmajorhistocompati
3、bilitycomplex(MHC)antigenswereusedtocharacterizeIFNaproducercells.TheproductionofIFNawasfoundtobeafunctionofnon-phagocytic,non-adherent,non-T,non-B,CD4+(andtoalesserextentCD8+)MHC-class-II-positiveceils.Fur-thermore,additionofanti-MHC(classII)mAbduringPBMCincubationwithviru
4、s-infectedcellsreducedIFNyields,suggestingthatmaskingofthesesurfaceantigensaltersPBMCresponsivenesstoIFNinduction.KEY-WORDS:Interferon-a,Leukocyte,MHC,Coronavirus;Transmissiblegastroenteritisvirus,mAb,PBMC.INTRODUCTIONPeripheralbloodleukocyteshavebeenshowntoproduceinterfero
5、nalpha(IFNa)uponinductionbyviruses,bacterialproductsortumourcells(Dian-zaniandCapobianchi,1987).IncontrasttoIFNbeta,forwhichthecriticalinducingfactorappearstobeviralRNA,adifferentmechanismseemstobeinvolvedininductionofIFNa.Thus,inactivatedvirusparticles,virus-infectedcells,
6、mycoplasmasandcellmembranesarecapableofinducingIFNa(Lebonetai.,1982;HughesandBlalock,1983;Gobletal.,1988;Capobianchietal..,1987),whichsuggeststhatmembraneinteractionsbetweentheinducerstructureandtheleukocytemembraneissufficienttotriggerIFNaproduc-142B.CHARLEYANDL.LAVENANTti
7、on.Inthecaseoftransmissiblegastroenteritisvirus(TGEV),acoronaviruswhichinducesacutediarrhoeaandintenseIFNsynthesisinnewbornpiglets(LaBonnardiereandLaude,1981),wehavepreviouslyshownthatearlyIFN~productioncouldresultfromexposureofnon-immunepiglymphocytestovirus-infectedcells.
8、Moreover,experimentsinwhichIFN0cproductionwasinhibitedbymonoclonalantibodies(mAb)d
