hematopoetic stem cells BLOOD

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1、PlenarypaperDynamictrackingofhumanhematopoieticstemcellengraftmentusinginvivobioluminescenceimagingXiuliWang,MichaelRosol,ShundiGe,DenisePeterson,GeorgeMcNamara,HarveyPollack,DonaldB.Kohn,MarvinD.Nelson,andGayM.CrooksThestandardapproachtoassesshemato-patt

2、ernsofengraftmentkineticsfromhu-miceafterdeathdemonstratedthatlevelspoieticstemcell(HSC)engraftmentinmanCD34
andCD34
CD38populationsof1%donorcellengraftmentcouldbeexperimentalbonemarrowtransplanta-inthemarrowNOD/SCID/2mnullmice.Imag-readilydetectedbybio

3、luminescenceim-tionmodelsreliesondetectionofdonoringshowedanearlypeak(day13)ofen-aging;higherengraftmentlevelscorre-hematopoieticcellsinhostbonemarrowgraftmentfromCD34
cellsfollowedbyaspondedtohigherimagesignalintensity.followingdeath;thisapproachprovides

4、rapiddeclineinsignal.EngraftmentfromInvivobioluminescenceimagingpro-datafromonlyasingletimepointafterthemoreprimitiveCD34
CD38popula-videsanovelmethodtotrackthedynam-transplantationforeachanimal.Invivotionwasrelativelydelayedbutbyday36icsofengraftmentofh

5、umanHSCandbioluminescenceimagingwasthereforeincreasedtosignificantlyhigherlevelsprogenitorsinvivo.(Blood.2003;102:exploredasamethodtogainadynamic,thanthosefromCD34
cells(P<.05).3478-3482)longitudinalprofileofhumanHSCengraft-SignalintensityfromCD34
CD38-men

6、tinalivingxenogeneicmodel.Lucif-engraftedmicecontinuedtoincreasedur-eraseexpressionusingalentiviralvectoringmorethan100daysofanalysis.Flowalloweddetectionofdistinctlydifferentcytometryanalysisofbonemarrowfrom©2003byTheAmericanSocietyofHematologyIntroducti

7、onKeyaspectsofthebiologyofhematopoieticstemcells(HSCs)Wenowshowthatbyaccomplishinghighefficiency,stablehavebeenstudiedformanyyearsusingelegantmurinemodelsoftransduction,andexpressionofluciferaseinHSCs,biolumines-bonemarrowtransplantation.Morerecently,immun

8、odeficientcenceimagingcanrevealnovel,dynamicinformationonthemurinemodelshavebeenusedsuccessfullytostudyhumanHSCs.1patternofhumanHSCandprogenitorengraftmentandprolifera-Inallofthesemodels,HSCsareinfusedintorecipientan