5.30 Expansion of Hematopoietic Stem=Progenitor Cells

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1、5.30ExpansionofHematopoieticStem/ProgenitorCellsTLiu,DalianUniversityofTechnology,Dalian,China©2011ElsevierB.V.Allrightsreserved.5.30.1Introduction3895.30.2SourcesofHSPCs3905.30.3RequirementofExpansionFoldsandQualityofHSPCs3905.30.4ExpansionofHSPCsunderCommonStaticCultureConditio

2、n3915.30.5ExpansionofHSPCsunderDynamicBioreactorCultureConditions3915.30.5.1PerfusionBioreactor3915.30.5.2StirredBioreactors3925.30.5.3Packed-andFluidized-BedBioreactors3925.30.5.4RotatingWallVessels3935.30.5.5OtherBioreactorTypes3935.30.6MimickingtheInVivoMicroenvironmenttoExpan

3、dHSPCs3935.30.6.1BMEnvironmentorNiche3935.30.6.2Co-culturewithStromalCells3945.30.6.3HSPCsCulturedin3DScaffolds3945.30.6.4HSPCExpansionwithMultiparameterComprehensiveMimickedEnvironment3955.30.7BriefIntroductionofClinicalApplicationTestswithExpandedHSPCs3955.30.8Summary396Referen

4、ces396GlossaryHSPCsexpansionAprocedurewherethecellnumberof++CD34cellsCellsexpressingCD34(CD34cell)arehematopoieticstem/progenitorcellsincreased,whiletheynormallyfoundintheumbilicalcordandbonemarrowaswereculturedintwo-dimensionalorthree-dimensionalhematopoieticcells,asubsetofmesen

5、chymalstemcells.culturesystems.graftMaterial,especiallylivingtissueoranorgan,stemcellnicheAstem-cellnicheisarestrictedlocalesurgicallyattachedtoorinsertedintoabodilypartinanorganthatsupportstheself-renewingdivisiontoreplaceadamagedpartorcompensateforadefect.ofstemcellsandsopreven

6、tsthemfromhematopoieticstem/progenitorcells(HSPCs)differentiating.Hematopoieticstem/progenitorcells(HSPCs)arestromalcellsThesecellsareconnectivetissuecellsofanmultipotentcellsthatgiverisetoallthebloodcelltypes,organfoundinthelooseconnectivetissue.includingmyeloidandlymphoidlineag

7、es.5.30.1IntroductionHematopoieticstem/progenitorcells(HSPCs)arethosemultipotentcellsabletoself-renewandgenerateallbloodcelllineages[1].HSPCshaveextensivepotentialapplicationsinclinicalapplications,includingstemcelltransplantation,geneandimmunotherapy,andthefabricationofmatureblo

8、odcells.Hematopoieticstemcelltransplanta