5.29 Induced Pluripotent Stem Cells and Their Application

《5.29 Induced Pluripotent Stem Cells and Their Application》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、5.29InducedPluripotentStemCellsandTheirApplicationtoPersonalizedTherapySACowley,UniversityofOxford,Oxford,UK©2011ElsevierB.V.Allrightsreserved.5.29.1Introduction3785.29.2hiPSCsAreSimilarto,butNotIdenticalto,hESCs3785.29.3GeneratinghiPSCs3795.29.3.1InformedConsent3795.29.3.2GoodMan

2、ufacturingPractice3795.29.3.3OperatorSafety3795.29.3.4ReprogrammingOptions3805.29.3.4.1Startingmaterial3805.29.3.4.2Reprogrammingfactors3805.29.3.4.3Polycistronicvectors3805.29.3.4.4Integratingvectors3805.29.3.4.5Excisablesystems3815.29.3.4.6Nonintegratingsystems3815.29.3.4.7Prote

3、inormRNA-only3815.29.3.4.8Smallmolecules3815.29.3.4.9Feederversusfeeder-freeculture3815.29.3.5ReprogrammingProtocols3815.29.3.5.1Seedingdensityandsplittingduringreprogramming3815.29.3.5.2Colonyidentification3825.29.3.5.3Successfulpassaging,expansion,andbankingofcolonies3825.29.3.5

4、.4CharacterizinghiPSClines3825.29.3.5.5Differentiationassays3835.29.4GeneticManipulationofhiPSCs3835.29.5GeneratingDifferentiatedCellPopulations3835.29.6TransplantationofhiPSC-DerivedCells3855.29.7PatientSafety3855.29.7.1ExperiencefromGeneTherapyandStemCellClinicalTrials3855.29.7.

5、2EngineeringSafetyNets3865.29.7.2.1Preventionofreversiontopluripotency3865.29.7.2.2Eliminationofwaywardcells3865.29.7.3BypassinghiPSCFormation3865.29.8Conclusion387References387GlossaryhematopoieticstemcellscandifferentiatetoallthecellsofdirecteddifferentiationDifferentiationalong

6、adefinedthehematopoieticlineage.pathwayusingapredeterminedcombinationof(usually)pluripotencyTheabilityofanEScelltodifferentiatetospecificcytokinesandmatrixcomponentscellsofallthreegermlayers(ecto-,meso-,andendoderm)embryoidbodyTheinitialstageofdifferentiationwhenandtheirdifferenti

7、atedprogeny.embryonicstem/inducedpluripotentstem(ES/iPS)reprogrammingfactorsFactorsusedtoreprogramcoloniesareliftedandspontaneouslyballup,formingsomaticcellstoapluripotentstate.Thisiscurrentlyecto-,meso-,andendodermallineagecells.acombinationoftranscriptionfactorgenesthathumanembr

8、yonicstemcell(hESC)Cellsderivedfo