冠状病毒Coronavirusl论文-1991 Glycosylation is Required for Coronavirus TGEV to Induce an Efficient Production of IFN_ by Blood Mononuclear Cells.pdf

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1、AtX)Nts0300947591(X)O53P.Scand.J.Immunol.33,435-440.1991GlycosylationisRequiredforCoronavirusTGEVtoInduceanEfficientProductionofIFNabyBloodMononuciearCellsB.CHARLEY.L.LAVENANT&B.DELMASLaboratoiredeVirologieetd'ImmunologicMoleculaires,LN.R.A.,CentredeReehcrehesde

2、Jouy-en-Josas,DomainedeVilvert.Jouy-cn-Josas.FranceCharley.B.Lavenant.L.&Delma.s.B.GlycosylationisrequiredforCoronavinisTGEVtoInduceanBllicientProductionoflFNxbyBloodMononuciearCells.SeimtfJ.Immunot.33,435440.1991.Porcineperipheralbloodmononuciearcells(PBMClarei

3、nducedtoproduceinterferonalpha(IFNa)followinginvitroexposuretoeoronavirusTGEV(transmissiblegastroenteritisvirus)-infectedglutaraldehyde-fixedcellmonolayersorloTGEVvirions.Inthepresentreporl.weexaminedthepossibilitythatglycosylationofviralproteinscouldplayamajorr

4、oleininteractionswithPBMCleadingtotheproductionol"IFN-i.ConApretreatmentofTGEV-infectedcellmonolayersbeforefixationwithglutaraldehydeandexposuretoPBMCcausedadosc-dcpendentinhibitionofIFN:einduction,implyingthatmaskingofcarbohydratesatthesurfaceofinfectedcellslow

5、eredIFN-a-induciion.Similarly,inhibitionofN-linkedglycosylationbytunicamycinduringviralinfectionofcellmonolayersalteredtheirabilitytoinduceIFNa.Inaddition,completecleavageof'complextype'oligosaccharidesbypcptide-N-glycohydrolaseFloweredthecapacityofTGEVvirionsto

6、induceIFNx.Thus,thesetindingsstronglysuggestthatglycosylationofthevira!proteins,andmorepreciselythepresenceofcomplex-typeoligosacehar-ides,isanimportantrequirementforacompletelyellicientinteractionwithPBMCleadingtotheproductionofIFN-a,B.Ctjartey.LahoratnircdeV'i

7、rotogieetdlmmunotogteMoteeutaires.I.N.R.A..CentredeReetieretiesdeJouv-en-Jti.as.DomtiinedeVitven.7fi..15OJiiii-en-Josas.FranceVariousstimulisuchasviruses,bacterialpro-natureoftheviralcomponentinvolvedinmem-ductsortumourcellscaninduceleucocytestobraneinteractio

8、nswithlymphocytesleadingtoprodticeinterferonalpha(IKNx)[7].Thedemon-theactivationofIFN-a-codinggenes.Thus,instrationthaiinactivatedvira!particlesorgluiar-thecourseofs