冠状病毒Coronavirusl论文-1991 Carbohydrate-induced conformational changes strongly modulate the antigenicity of coronavirus TGEV glycoproteins S.pdf

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1、VirusResearch,20(1991)107-12010701991ElsevierSciencePublishersB.V.016%1702/91/$03.50ADONIS0168170291000962VIRUS00675Carbohydrate-inducedconformationalchangesstronglymodulatetheantigenicityofcoronavirusTGEVglycoproteinsSandMBernardDelmasandHubertLaudeLaboratoiredeViroogieetdlmmunologieMol

2、t%ulaires,InstitutNationaldelaRechercheAgronomique,Joey-en-Josas,France(Accepted2April1991)Summa~ThecarbohydratecompositionandtheimmunoreactivityoftheSandMglycoproteinsofthecoronavirusTGEVwerestudiedatdifferentstagesoftheirmaturation.ThebiosynthesisofSandMwasanalyzedinthepresenceoftunica

3、mycinandmonensin.TheeffectoftreatmentwithendoglycosidasesHandFandglycopeptidaseFontheprecursorsandmatureformsofSandMwerealsoexamined.Species175Kand29KwerecharacterizedashighmannoseformsofSandM,respectively,andspecies220Kand30-36Kascomplextypeglycosylatedformsofthesetwoproteins.Mwaspresen

4、tmainlyasa29Kspeciesinmaturevirionswhereasthe175KformofSwasnotdetected,thusimplyingthatthetwoproteinsundergoGolgimodificationsatafardifferentefficiency.Anti-Sand-MmonoclonalantibodieswereexaminedfortheirreactivitytowardspolypeptidespecieseithertreatedwithendoHorproducedinthepresenceoftun

5、icamycin.Itwasfoundthat(i)amongthefourmajorantigenicsitespreviouslydefined(Delmasetal.,1986),onlysiteC(aminoacids363to371)wasnotablyexpressedbytheunglycosylatedSpolypeptide155K,whereasthethreeothersitesweredependentuponore-gIycosylation,(ii)threeofthefouranti-MmAbstesteddidnotrecog-nizet

6、heunglycosylatedMpolypeptide26K.Thesedataledustoconcludethatco-translational,butnotterminalglycosylationisanessentialrequirementforbothacquisitionandmaintenanceoftheantigenicityofTGEVglycoproteins.C#~re~~ndenceto:H.Laude,LaboratoiredeVirologieetdImmunologieMol~culaires,InstitutNationalde

7、laRechercbeAgronomique,78352Jouy-en-Josascedex,France.108Coronavirus;Glycoprotein;Glycosylation;Transmissiblegastroenteritisvirus;TGEV;Antigenicity;EpitopeIntroductionCoronavirusesareenvelopedviruseswithalargepositive-strandedRNAgenome.Thevirionscontainbasicallytwoenvelop