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1、PublishedSeptember1,1987ASpecificTransmembraneDomainofaCoronavirusE1GlycoproteinIsRequiredforItsRetentionintheGolgiRegionCarolynE.MachamerandJohnK.RoseMolecularBiologyandVirologyLaboratory,TheSalkInstituteforBiologicalStudies,SanDiego,California92138Abstract.TheE1gly
2、coproteinoftheaviancoronavi-canfunctionasaninternalsignalsequence.Themu-msinfectiousbronchitisviruscontainsashort,tantproteinwithonlythefirsttransmembranedomainglycosylatedamino-terminaldomain,threemembrane-accumulatedintracelluladylikethewild-typeprotein,spanningdom
3、ains,andalongcarboxy-terminalcyto-butthemutantproteinwithonlythethirdtransmem-plasmicdomain.WeshowthatE1expressedfrombranedomainwastransportedtothecellsurface.ThisDownloadedfromcDNAistargetedtotheGolgiregion,asitisinin-resultsuggeststhatinformationspecifyingaccumula-
4、fectedcells.E1proteinswithprecisedeletionsofthetionintheGolgiregionresidesinthefirsttransmem-firstandsecondorthesecondandthirdmembrane-branedomain,andprovidesthefirstexampleofanin-spanningdomainswereglycosylated,thussuggestingtracellularmembraneproteinthatistransport
5、edtothethateitherthefirstorthirdtransmembranedomainplasmamembraneafterdeletionofaspecificdomain.jcb.rupress.orghEintracellulartransportandtargetingofproteinsteinswiththesesignalswouldbetransportedtothecellsur-fromtheirsiteofsynthesistotheircorrectdestinationfaceviaat
6、ypeofreceptor-mediatedprocess,likethatTisaprocessinstrumentaltomalntainenceofcellularinvolvedintargetingoflysosomalenzymes.Alternatively,asintegrity.Membrane-boundandsecretedproteinsaresortedrecentlysuggestedbyKelly(1985)andRothman(1986),pro-fromcytoplasmicproteinsan
7、dthosedestinedforthenucleusteinsthataredestinedfortheplasmamembraneorforcon-onJune4,2015ormitochrondriabyvirtueofasignalorleadersequencestitutivesecretioncouldmovepassivelywiththebulkflowwhichdirectsinsertionintothelumenoftheendoplasmicoflipids,whileproteinssecretedi
8、naregulatedmannerorreticulum(Walteretal.,1984).Plasmamembraneandse-thoseretainedinintracellularmembraneswouldpossesssig-cretedprote
