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1、内蒙古农业科技2015,43(5):7~9InnerMongoliaAgriculturalScienceAndTechnology玉米秸秆低温降解菌系的生长特性及其降解效果的研究高琳1111111112,高聚林,闹干朝鲁,王振,于晓芳,胡树平,萨如拉,青格尔,胡海红,赵丽萍(1.内蒙古农业大学农学院,内蒙古呼和浩特0100192.鄂尔多斯市植保植检站,内蒙古东胜017000)摘要:探讨玉米秸秆低温降解复合菌系GF-S72在液态发酵条件下的生长特性及其降解效果。结果表明:在10℃条件下,施加复合剂GF-S72低温条件下提高玉米秸秆的
2、降解效果。复合菌系的生长曲线在接菌后直接进入对数期,11d时生长量最大,其OD值为0.246;pH值在培养初期迅速下降,随后稳定在7.2左右;FPA,C1,CX,Cb酶活菌先升高后下降,最高酶活分别为0.82,1.41,1.33,1.25U/mL;培养15d降解率为34.70%,显著高于CK;纤维素、半纤维素、木质素残余量分别为1.12g,0.72g,0.27g,显著高于CK;因此复合菌系GF-S72是十分具有研究开发潜力的复合菌系。试验结果为该菌进一步开发玉米秸秆还田低温降解复合菌剂提供试验参数和依据。关键词:降解菌系;低温;玉米
3、秸秆中图分类号:Q939.96;S513文献标识码:A10.3969/j.issn.1007-0907.2015.05.002文章编号:1007-0907(2015)05-0007-03Theresearchofthegrowthcharacteristicsanddegradationoflow-temperaturecornstrawsdegradationcompoundstrainGAOLin,GAOJulin,NAOGANChaolu,WANGZhen,YUXiaofang,HUShuping,SARula,QINGGee
4、r,HUHaihong,ZHAOLiping(InnerMongoliaAgriculturalUniversity,Hohhot010019,China)Abstract:Discussthelow-temperaturedegradationofcornstrawscompoundstrainsgrowthcharacteristicsanddegradationinliquidfermentationconditionscalledGF-S72.Theresultsshowed:under10℃condition,GF-72c
5、ouldimprovetheeffectsonthedegradationofcornstraws.Thegrowthcurveofcompoundstrainsdirectlyintothelogarithmicphaseafterinoculation,the11dlargestgrowth,theODvalueof0.246;pHvaluedecreasedrapidlyintheearlystageofincubation,andthenstabilizedataround7.2;degradationrateof34.70
6、%after15d,significantlyhigherthanCK;cellulose,hemicellulose,ligninremainingamountwas1.12g,0.72g,0.27g,wassignificantlyhigherthanCK.ThereforthereforeGF-S72complexstrainshasaresearchanddevelopmentpotentialisverycomplexstrains.SocompoundstrainnamedGF-S72wasastrainwithhadt
7、hepotentialofresearchanddevelopment.Theresultshowedthatthetestparametersandbasiswereprovidedtofurtherdevelopstrawcounters-fieldcompoundbacteriumagentforthebacteria.Keywords:Degradablemicrobes;Lowtemperature;Cornstalks作为一个农业大国,我国每年会产生7亿t农作在干旱、寒冷条件下,玉米秸秆降解受到多方面物秸秆,但实际利用率
8、相对较低[1][2-4]等对我因素的影响,自然腐解速度极为缓慢。研究表明,在纤。高利伟国农作物秸秆去向的研究表明,秸秆还田约占总量的维素物质中,分子间与分子内的氢键给纤维素水解带来很大的困难[5]24.3%;饲用约占总量的29.9%;