单分子检测技术.doc

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1、伍中山Futurelab-on-a-chiptechnologiesforinterrogatingindividualmolecules2010-10-19Backgroundhighthroughput&lowcostSamplingdemandhighresolution&manipulationMicroscopyTechnologiesLabelMaterial-processingOutlineintegrationSingleprobeMolecularimagingandprobesImagingProbesChip-basedsystemDevel

2、opingtechnologiesforlab-on-a-chipTechnologiesMaterialprocessingFluidicsBiochemicalpatterningMiniaturizedopticalsources&detectorsElectrical&MechanicaldevicesMaterialrequirementsSiliconcompounds/polymers/porousmediabElectrodearrayadElectricalchipswithhighspatialresolutioncElectricalchips

3、withhighspatialresolutionPatterningofactivebiomaterialsdPlannardevicesforsingle-moleculeobservationMagneticbeadbasedmolecularmotorPlannardevicesforsingle-moleculeobservationNanoporesusedforDNAtranslocationstudies.Single-moleculeopticalanalysisinflowingsystemsAnalyteislabeledwith:Fluorescentdye

4、moleculesOrluminescentparticlesUsedto:countmoleculesdetectsinglebindingeventsmeasureelectrophoreticmobilitySingle-moleculeopticalanalysisinflowingsystemsNanofluidicsystemstoinvestigatephysicalpropertiesofindividualmoleculesOutlook105〜106103SinglemoleculesisolatedscaleFluidsystemsnaturalbiologi

5、calsystems+integration"Superchip”Opticalbeams+ElectricaltechniquesOpportunitiesforsinglemoleculeapproachesToanalyzesmallvolumesofcomplexmixturesTohandlecellsandpresentcontrolledstimulitocellsBiologicalapplicationMedicalapplicationdiagnosisMonitoringoftherapies