支原体污染的预防.ppt

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1、BIOLOGICALINDUSTRIESDOYOUSUSPECTMYCOPLASMA?Prevention,DetectionandTreatmentofMycoplasmaContamination支原体污染的预防，检测与治疗BiologicalIndustriesoffersyou:MYCOPLASMADETECTION支原体检测EZ-PCRMycoplasmaTestKitCat.#20-700-10(10assays)Cat.#20-700-20(20assays)ReadytousePCRmixforthedetectionofmycoplasmaincellcu

2、lture即用型细胞培养中支原体检测PCR试剂盒EZ-PCRMycoplasmaTestKitisdesignedtodetectthepresenceofmycoplasmacontaminatingbiologicalmaterials,suchasculturedcells.EZ-PCR支原体检测试剂盒用来检测生物材料，如培养的细胞中支原体的存在。Mycoplasmadetectionbythedirectcultureprocedureistime-consumingandsomemycoplasmaspeciesaredifficulttocultivate.直接

3、培养方法检测耗时且有的种类支原体很难培养。WithPCRtesting,resultsareobtainedwithinafewhours,sincethepresenceofcontaminantmycoplasmacanbeeasilydetectedsimplybyverifyingthebandsofamplifiedDNAfragmentsinelectrophoresis.用PCR检测，几小时可出结果。支原体DNA片段可通过电泳条带确认。Theprimersetallowsdetectionofvariousmycoplasmaspecies,withhighs

4、ensitivityandspecificity:系列引物可以高特异性和高灵敏度地检测很多种支原体TREATMENTOFMYCOPLASMA-INFECTEDCELLSWITHANTIBIOTICS用抗生素治疗支原体污染的细胞Thecontaminationofcellswithmycoplasmaisaverycommonproblem,eventhoughitoftengoesunnoticedsincenocloudinessappearsinthecellculture.支原体污染细胞培养是很常见的问题，常常因其不造成混浊而不被注意到。Sincemycoplasma

5、-infectedcellscannotalwaysbediscarded,manycomplicatedmethodshavebeensuggestedfortheeliminationofthemycoplasma.支原体污染的细胞有时不能废弃，有许多复杂的方法可以用来去除污染。BiologicalIndustriesisnowofferingacombinationofantibiotics,whichhavebeenshowntobeeffectiveintheeliminationofmycoplasmaspeciesthataccountfor90%ofthec

6、ontaminationfoundincellcultures.BiologicalIndustries的抗生素系列产品能有效杀灭细胞培养中的90%以上的支原体BIOMYC-3(cat.#03-038-1)BIOMYC-1(cat.#03-036-1)&BIOMYC-2(cat.#03-037-1)BIOMYC-1isbasedontheantibiotictiamutin,whichisproducedbythefunguspleurotusmutilus.成分为泰妙菌素.BIOMYC-2isbasedonminocycline,whichisatetracyclined

7、erivative.二甲胺四环素，四环素衍生物.Thesetwoantibioticsolutionsaregenerallyusedsequentiallyincombination.以上抗生素溶液一般按顺序联合使用.BIOMYC-1&BIOMYC-2BIOMYC-3BIOMYC-3isbasedontheciprofloxacinantibiotic,whichisamemberofthefluoroquinolonegroup.Manymycoplasmaspecieshavebeenfoundtobes