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1、小鼠子宫内膜的组织培养【关键词】子宫内膜InvitrocultureofmouseendometriumTANDongMeil,TANYil,ZHAOJie2,AlLing2,WANGZhiBiao2lLaboratoryAnimalCenter,2UltrasoundEngineeringInstituteofMedicalScience,ChongqingUniversityofMedicalScience,Chongqing400016,China[Abstract】AIM:Todevelopaninvitroculturemodelofmouseendometr
2、ium・METHODS:MouseendometriumsqueezedfromtheuterushornswereculturedatthegasliquidinterfacebetweenF12/DMEM(1：1)including200inL/LFBS,17.5nmol/Linsulin,63.5nmol/Lprogesterone,7.14nmol/Lestradiol,and50mL/LC02plus950mL/Lair・Theeffectsof20ug/Lepidermalgrowthfactor(EGF)andthoseofmixedgascontaini
3、ng50mL/LC02plus950mL/L02onthemaintenanceoftheculturedendometriumwerecompared・Thenecrosisrateofthesampleswasmeasuredafterhematoxylineosinstainingbyusingtheimageanalysissystem・Theexpressionsofleukemiainhibitoryfactor(L1F)andheparinbindingepidermalgrowthfactorlikegrowthfactor(HBEGF)inthemou
4、seendometriumweredetectedbyimmunohistochemistrytodeterminewhethertheendometriumculturedfor24hwasstillreceptivetotheblastocysts・RESULTS:Thenecrosisrate(%)oftheendometriuminthethreegroupswas19.40土3.38,16.41土3.40and13.71+2.89respectively,showingasignificantdifference(P=0.000).Thepositivelyr
5、atesofLIFandHBEGFproteinsinbothculturedandcontrolledmouseendometriumwere100%unexceptionallyandtherewasnosignificantdifferenceinthestainingintensityforeachmarkerbeforeandaftertheculture(P二0.237,0.224)・CONCLUSION:Theculturecondition:F12/DMEM(1:1),200mL/LFBS,17.5nmol/Linsulin,63.5nmol/Lprog
6、esterone,7.14nmol/Lestradiol,20Pg/LEGFand50mL/LC02plus950mL/L02,provestobemostoptimalforthegrowthofmouseendometriuminvitro.Theendometriumculturedfor24hoursisstillreceptivetotheblastocysts・[Keywords】endometrium;culture;leukemiainhibitoryfactor;heparinbindingepidermalgrowthfactorlikegrowth
7、factor【摘要】目的：建立小鼠子宫内膜的体外培养方法.方法：用挤压法获得子宫内膜,在添加200inL/L胎牛血清(FBS),17.5nmol/L胰岛素，63.5nmol/L孕酮(P4),7.14nmol/L雌二醇(E2)的F12/DMEM(1:1)培养液和50mL/LC02+950mL/L空气界面进行培养，比较分析20ug/L表皮生长因子(EGF)和50mL/LC02+950mL/L02气体条件对子宫内膜维持的影响.HE染色，图像分析系统检测样本坏死率，并用SP法检测培养前后子宫内膜中白血病抑制因子(LIF)及类肝素结合样表皮生长因子
