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1、AdvancePublicationTheJournalofVeterinaryMedicalScienceAcceptedDate:6May2018J-STAGEAdvancePublishedDate:17May20181FullPaper,Virology2MutationoftheSand3cgenesingenomesoffelinecoronaviruses31)1)1,2)1)4KeisukeOguma*,MegumiOhno,MayukoYoshidaandHiroshiSentsui51)6LaboratoryofVeterinaryEpizootiology,Depar
2、tmentofVeterinaryMedicine,College7ofBioresourceSciences,NihonUniversity,1866Kameino,Fujisawa,Kanagawa252-80880,Japan2)9Presentaddress:OkinawaZooandMuseumFoundation,5-7-1Goya,OkinawaCity,10Okinawa904-0021,Japan1112*Correspondingauthor:Oguma,K.,LaboratoryofVeterinaryEpizootiology,13DepartmentofVeter
3、inaryMedicine,NihonUniversity,1866Kameino,Fujisawa,14Kanagawa252-0880,Japan.15E-mail:oguma.keisuke@nihon-u.ac.jp16Tel/Fax:+81-466-84-33681718RunningHead:MUTATIONOFFELINECORONAVIRUSGENOME19120ABSTRACT21Felinecoronavirus(FCoV)isclassifiedintotwobiotypesbasedonitspathogenicity22incats:afelineentericc
4、oronavirusoflowpathogenicityandahighlyvirulentfeline23infectiousperitonitisvirus.IthasbeensuspectedthatFCoValtersitsbiotypevia24mutationsintheviralgenome.TheSand3cgenesofFCoVhavebeenconsideredthe25candidatesforviralpathogenicityconversion.Inthepresentstudy,FCoVswere26analyzedforthefrequencyandloca
5、tionofmutationsintheSand3cgenesfromfaecal27samplesofcatsinananimalshelterandthefaeces,effusions,andtissuesofcatsthat28werereferredtoveterinaryhospitals.Ourresultsindicatedthatapproximately95%29FCoVsinfaecesdidnotcarrymutationsinthetwogenes.However,80%FCoVsin30effusionsamplesexhibitedmutationsinthe
6、Sand3cgeneswithremainderdisplayinga31mutationintheSor3cgene.Itwasalsosuggestedthatmutationalanalysisofthe3c32genecouldbeusefulforstudyingthehorizontaltransmissionofFCoVsinmulti-cat33environments.3435KEYWORDS:felinecoronavirus,multi-catenvironment,mutation,Sgene,3cgene,36237INTRODUCTION38Thegenomeo
7、ffelinecoronavirus(FCoV),amemberoftheAlphacoronavirus139speciesofthegenusAlphacoronavirus,comprisessingle-strandedpositive-senseRNA40[9].FCoVinfectionisprevalentincatsworldwideandisdividedintotwobiotypes:41feline
